Tobia M H, Zdanowicz M M, Wingertzahn M A, McHeffey-Atkinson B, Slonim A E, Wapnir R A
Department of Pediatrics, North Shore University Hospital-New York University School of Medicine, Manhasset 11030, USA.
Mol Genet Metab. 1998 Mar;63(3):205-13. doi: 10.1006/mgme.1997.2672.
The goal of this study was to determine whether zinc supplementation in the diet of diabetes-prone BB Wistar rats will delay or prevent the onset of overt diabetes. Male Wistar BB rats were fed diets containing either 1000 ppm (HZ), 50 ppm (NZ), or 1 ppm zinc (LZ) starting at 30 days of age. Non-diabetes-prone rats were fed NZ and designated as controls (NORM). Beginning at 60 days, the rats were checked for glycosuria and, if positive, were given an i.p. glucose tolerance test (IPGTT). All remaining animals underwent an IPGTT at 100 days and were sacrificed. At 90 days of age HZ rats had a lower incidence of diabetes (19%) than NZ (53%) or LZ (44%) animals (P < 0.015). By age 100 days, for the HZ group, there was a 60% reduction in the number of expected overt diabetic rats. HZ animals also had higher concentrations of both pancreatic and serum insulin and exhibited lower serum glucose and triglycerides. Immunohistochemistry of HZ rats was clearly different from NZ rats and showed evidence of nearly normal pancreatic endocrine activity. Data indicate that dietary treatment of diabetes-prone BB Wistar rats with zinc appears to be an effective approach for delaying or preventing the onset of diabetes in genetically predisposed rodents. This finding may suggest further experimental studies regarding dietary means for preservation of pancreatic function.
本研究的目的是确定在易患糖尿病的BB Wistar大鼠饮食中补充锌是否会延迟或预防显性糖尿病的发生。从30日龄开始，给雄性Wistar BB大鼠喂食含1000 ppm（高锌组，HZ）、50 ppm（正常锌组，NZ）或1 ppm锌（低锌组，LZ）的饲料。非易患糖尿病的大鼠喂食NZ饲料并指定为对照组（正常组，NORM）。从60日龄开始，检查大鼠是否有糖尿，若呈阳性，则进行腹腔内葡萄糖耐量试验（IPGTT）。所有剩余动物在100日龄时进行IPGTT并处死。90日龄时，HZ组大鼠的糖尿病发病率（19%）低于NZ组（53%）或LZ组（44%）动物（P < 0.015）。到100日龄时，HZ组预期显性糖尿病大鼠的数量减少了60%。HZ组动物的胰腺和血清胰岛素浓度也更高，且血清葡萄糖和甘油三酯水平较低。HZ大鼠的免疫组织化学与NZ大鼠明显不同，显示出胰腺内分泌活动几乎正常 的证据。数据表明，用锌对易患糖尿病的BB Wistar大鼠进行饮食治疗似乎是一种有效方法，可延迟或预防遗传易感性啮齿动物糖尿病的发生。这一发现可能意味着需要进一步开展关于饮食手段对胰腺功能保护作用的实验研究。
