Pavlov A V, Kemoklidze K G
Yaroslavl State Medical Academy, Russia.
Ontogenez. 1998 Mar-Apr;29(2):123-8.
We have studied the contribution of proliferation and hypertrophy of glandular cells to the ontogenetic growth of adrenal chromaffin tissue using several methods (organometry, cytometry, cytophotometric quantitation of DNA in the nuclei, radioautographic analysis of 3H-thymidine incorporation, calculation of the mitotic index and proportion of binuclear cells, as well as stereological analysis). Mitotic division of diploid glandular cells is the main cellular mechanism of postnatal growth of chromaffinocytes. It is most prominent during the first 2 weeks of life and is maintained at a rather high level throughout the life of animals (the daily proliferative pool in 6-month-old and 30-month-old rats equals 0.3%). Development of cellular hypertrophy has been noted during the first 6 months after birth. The population of chromaffinocytes throughout life is practically diploid; the proportion of tetraploid (binuclear) cells does not exceed 1-1.5%. The growth of adrenal chromaffin tissue during the first month of life is generally supported by hyperplasia and hypertrophy of norepinephrocytes and later of epinephrocytes. The contribution of cell proliferation and hypertrophy to postnatal growth of each subpopulation appears to be equal.
我们使用了多种方法（器官测量法、细胞计数法、细胞核DNA的细胞光度法定量、3H-胸腺嘧啶核苷掺入的放射自显影分析、有丝分裂指数及双核细胞比例的计算以及体视学分析）研究了腺细胞增殖和肥大对肾上腺嗜铬组织个体发育生长的贡献。二倍体腺细胞的有丝分裂是嗜铬细胞出生后生长的主要细胞机制。它在出生后的头两周最为显著，并在动物的整个生命过程中维持在相当高的水平（6月龄和30月龄大鼠的每日增殖池均为0.3%）。在出生后的头6个月内已观察到细胞肥大的发展。嗜铬细胞群体在整个生命过程中实际上都是二倍体；四倍体（双核）细胞的比例不超过1 - 1.5%。出生后第一个月肾上腺嗜铬组织的生长通常由去甲肾上腺素能细胞以及后来的肾上腺素能细胞的增生和肥大所支持。细胞增殖和肥大对每个亚群出生后生长的贡献似乎是相等的。
