Gansburgskiĭ A N, Pavlov A V
Ontogenez. 1994 May-Jun;25(3):33-9.
Contribution of proliferation and hypertrophy of endotheliocytes to the postnatal growth of the internal aortic lining in rats was estimated using organo- and cytometry, cytophotometry of nuclear DNA, radioautographic analysis of 3H-thymidine incorporation, determination of the mitotic index and the number of binucleated cells. Mitotic divisions of diploid endotheliocytes (in adult rats, their proportion attains 97-98%) is the principal cytological mechanism that, throughout the life of an animal, provides for an increase in the area of endothelial layer. Cell hypertrophy, which is unrelated to polyploidization, develops only during the first month of postnatal life. In old rats (2.5 years of age), an increased proportion of binucleate cells (2c x 2) and cells with pycnotic nuclei is observed.
利用器官计量法和细胞计量法、细胞核DNA的细胞光度测定法、对3H-胸腺嘧啶核苷掺入的放射自显影分析、有丝分裂指数的测定以及双核细胞数量的测定，评估了内皮细胞增殖和肥大对大鼠主动脉内膜出生后生长的贡献。二倍体内皮细胞的有丝分裂（在成年大鼠中，其比例达到97%-98%）是主要的细胞学机制，在动物的整个生命过程中，这种机制使得内皮层面积增加。与多倍体化无关的细胞肥大仅在出生后的第一个月出现。在老年大鼠（2.5岁）中，观察到双核细胞（2c×2）和核固缩细胞的比例增加。
