Tukachinskiĭ S E, Tiagotin Iu V
Zh Mikrobiol Epidemiol Immunobiol. 1976 Jan(1):87-91.
Dynamics of specific binding of the antigen with rabbit blood lymphocytes in the process of immunization was studied by radioimmunochemical method with the use of human serum albumin, I125-labeled. Three types of response to the antigenic stimulant were revealed: biphasic with two maximal of the antigen-binding activity -- on the 5th--10th and the 15th--20th day; uniphasic -- with the maximum activity on the 15th--20th day, the absence of increase of the antigen-binding activity of blood lymphocytes and a high mean level of specific antigens; the absence of primary cell reaction to the administration of the antigen and low level of specific antibodies. A correlation was revealed between the intensity of the humoral response and the sum total amount of the immunocompetent lymphocytes entering the circulation in the course of the whole immunization period.
采用碘-125标记的人血清白蛋白，通过放射免疫化学方法研究了免疫过程中抗原与兔血淋巴细胞特异性结合的动力学。揭示了对抗抗原刺激的三种反应类型：双相反应，在第5至10天和第15至20天有两个抗原结合活性最大值；单相反应，在第15至20天有最大活性，血淋巴细胞抗原结合活性无增加且特异性抗原平均水平高；对抗原给药无原发性细胞反应且特异性抗体水平低。揭示了体液反应强度与整个免疫期间进入循环的免疫活性淋巴细胞总量之间的相关性。
