Doronkin S G, Korochkin L I
Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
Genetika. 1998 Apr;34(4):569-70.
In 1980, Belyaeva et al. found in Drosophila melanogaster the ecs (ecdysterone sensitivity) locus, later designated BR-C (Broad-Complex). This locus plays a key role in the reaction cascade of ecdysterone-regulated development. The gene BR-C was cloned by Chao and Guild (1986) and Belyaeva et al. (1987). To conduct "walking along the chromosome" in Drosophila virilis, cloned sequences of the BR-C gene from D. melanogaster were used as probes, hybridizing the start sites. A fragment of about 100 kb containing BR-C of D. virilis was cloned. A restriction map of the cloned region was created.
1980年，别利亚耶娃等人在黑腹果蝇中发现了ecs（蜕皮激素敏感性）基因座，后来将其命名为BR-C（泛复合体）。该基因座在蜕皮激素调节发育的反应级联中起关键作用。BR-C基因由赵和吉尔（1986年）以及别利亚耶娃等人（1987年）克隆。为了在粗壮果蝇中进行“染色体步移”，使用从黑腹果蝇克隆的BR-C基因序列作为探针，杂交起始位点。克隆了一段约100 kb包含粗壮果蝇BR-C的片段。构建了克隆区域的限制性图谱。
