Beacock-Sharp H, Young J L, Gaston J S
University of Cambridge School of Clinical Medicine, Department of Medicine, Addenbrooke's Hospital.
Ann Rheum Dis. 1998 Feb;57(2):100-6. doi: 10.1136/ard.57.2.100.
Reactive arthritis (ReA), a HLA-B27 associated arthropathy, develops in susceptible people after infection with certain bacteria. T cells have been implicated in the pathogenesis of the arthritis but which of the different subsets is involved is still debated. This study has further elucidated the role of the CD4+ and CD8+ T cells by examining the expression of various surface markers associated with activation.
Three colour flow cytometry was used to examine the phenotype of the T cells within the synovial fluid (SF) and peripheral blood (PB) of ReA patients.
ReA SF, compared with paired PB, contained a higher percentage of CD69+, CD25+, and HLA-DR+ CD3+ T cells. The majority of SF T cells also expressed the putative memory marker CD45RO. Within the T cell subsets, CD25 was expressed primarily on the CD4+ T cells; however more CD8+ T cells were HLA-DR+.
The results show that both CD4+ and CD8+ T cell populations demonstrate evidence of recent activation. Whether these cells are involved in inducing inflammation, regulating the inflammation, or have become active as a result of migration through the endothelium, remains to be determined by functional studies.
反应性关节炎（ReA）是一种与HLA - B27相关的关节病，在易感人群感染某些细菌后发病。T细胞被认为与关节炎的发病机制有关，但具体是哪一种不同的亚群参与其中仍存在争议。本研究通过检测与活化相关的各种表面标志物的表达，进一步阐明了CD4 +和CD8 + T细胞的作用。
采用三色流式细胞术检测ReA患者滑液（SF）和外周血（PB）中T细胞的表型。
与配对的PB相比，ReA SF中CD69 +、CD25 +和HLA - DR + CD3 + T细胞的百分比更高。大多数SF T细胞也表达假定的记忆标志物CD45RO。在T细胞亚群中，CD25主要表达于CD4 + T细胞；然而，更多的CD8 + T细胞表达HLA - DR。
结果表明，CD4 +和CD8 + T细胞群体均显示出近期活化的证据。这些细胞是参与诱导炎症、调节炎症，还是由于通过内皮迁移而变得活跃，仍有待功能研究来确定。
