Z'Graggen W J, Metz G A, Kartje G L, Thallmair M, Schwab M E
Brain Research Institute, University of Zurich and Swiss Federal Institute of Technology Zurich, CH-8029 Zurich, Switzerland.
J Neurosci. 1998 Jun 15;18(12):4744-57. doi: 10.1523/JNEUROSCI.18-12-04744.1998.
After a lesion of the mature CNS, structural plasticity and functional recovery are very limited, in contrast to the developing CNS. The postnatal decrease in plasticity is correlated in time with the formation of myelin. To investigate the possible role of an important myelin-associated neurite growth inhibitor (NI-250; IN-1 antigen), one pyramidal tract of adult Lewis rats was lesioned (pyramidotomy), and the rats were treated with the antibody IN-1, a control antibody, or no antibody. Functional recovery was studied from postoperative day 14 until day 42 using a food pellet reaching task, rope climbing, and a grid walk paradigm. The corticofugal projections to the red nucleus and basilar pontine nuclei were analyzed after survival times of 2 and 16 weeks. Treatment with the monoclonal antibody IN-1 resulted in almost complete restoration of skilled forelimb use, whereas all the control groups showed severe and chronic impairments. This functional recovery was paralleled by sprouting of the corticorubral and the corticopontine fibers across the midline, thus establishing a bilateral, anatomically specific projection.
与发育中的中枢神经系统（CNS）相比，成熟的CNS受损后，结构可塑性和功能恢复非常有限。出生后可塑性的降低在时间上与髓鞘的形成相关。为了研究一种重要的髓鞘相关神经突生长抑制剂（NI-250；IN-1抗原）的可能作用，对成年Lewis大鼠的一条锥体束进行损伤（锥体束切断术），然后用抗体IN-1、对照抗体或不使用抗体对大鼠进行治疗。从术后第14天到第42天，使用食物颗粒获取任务、爬绳和网格行走范式研究功能恢复情况。在存活2周和16周后，分析皮质向红核和脑桥基底核的投射。用单克隆抗体IN-1治疗导致熟练前肢使用几乎完全恢复，而所有对照组均表现出严重和慢性损伤。这种功能恢复与皮质红核纤维和皮质脑桥纤维越过中线的发芽同时发生，从而建立了双侧、解剖学上特定的投射。
