Stuart K, Kable M L, Allen T E, Lawson S
Seattle Biomedical Research Institute, Washington 98109-1651, USA.
Methods. 1998 May;15(1):3-14. doi: 10.1006/meth.1998.0601.
RNA editing in trypanosomes produces mature mRNAs by posttranscriptional guide RNA (gRNA)-directed uridylate insertion and deletion. This article describes methods for the study of RNA editing with an emphasis on an in vitro editing system that was used to explore the general mechanism of editing and that can be adapted for more in-depth studies of this intriguing and important process. Methods used to investigate the macromolecular complex that catalyzes RNA editing are also described. This complex is composed of multiple proteins and contains several catalytic activities. It is in the early stages of characterization. The methods described here are intended to assist in its further analysis.
锥虫中的RNA编辑通过转录后引导RNA（gRNA）指导的尿苷酸插入和缺失产生成熟的mRNA。本文介绍了RNA编辑的研究方法，重点介绍了一种体外编辑系统，该系统用于探索编辑的一般机制，并且可用于对这一有趣且重要的过程进行更深入的研究。文中还描述了用于研究催化RNA编辑的大分子复合物的方法。该复合物由多种蛋白质组成，并具有多种催化活性。其表征尚处于早期阶段。这里描述的方法旨在帮助对其进行进一步分析。
