用于麻风病检测及麻风病化疗疗效评估的DNA扩增技术
DNA amplification for detection of leprosy and assessment of efficacy of leprosy chemotherapy.
作者信息
Kampirapap K, Singtham N, Klatser P R, Wiriyawipart S
机构信息
Swellengrebel Laboratory of Tropical Hygiene, Royal Tropical Institute, Amsterdam, The Netherlands.
出版信息
Int J Lepr Other Mycobact Dis. 1998 Mar;66(1):16-21.
Polymerase chain reaction (PCR) for the detection of Mycobacterium leprae was applied to fresh skin biopsies and slit-skin smears from 122 untreated leprosy patients. The PCR positivity rates in biopsies were 95.6% in multibacillary (MB) cases and 44.2% in paucibacillary (PB) cases. Following 1 month of treatment, MB cases declined by 54.3% and PB cases by 61.8% of initial values. Six-month values also declined from initial positivity rates to 50.3% and 53.8% of initial values in MB and PB, respectively. Larger declines in the rate of positivity were seen for skin-smear samples at 1 and 6 months in both MB and PB, but overall PCR positivity rates were lower than biopsy rates for M. leprae.
采用聚合酶链反应（PCR）检测122例未经治疗的麻风患者的新鲜皮肤活检组织和皮肤刮片。多菌型（MB）病例活检组织的PCR阳性率为95.6%，少菌型（PB）病例为44.2%。治疗1个月后，MB病例下降至初始值的54.3%，PB病例下降至初始值的61.8%。6个月时，MB和PB病例的阳性率也分别从初始阳性率下降至初始值的50.3%和53.8%。MB和PB病例在1个月和6个月时，皮肤涂片样本的阳性率下降幅度更大，但总体上麻风杆菌的PCR阳性率低于活检组织的阳性率。
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