Lemmens I H, Kas K, Merregaert J, Van de Ven W J
Laboratory for Molecular Oncology, KU Leuven, Belgium.
Genomics. 1998 May 1;49(3):437-42. doi: 10.1006/geno.1998.5296.
In this report, the identification and molecular characterization of a novel gene, designated TM7SF2, is reported. This gene was found in the FAU neighboring area (FAUNA) to which other genes have been mapped previously. The FAUNA gene cluster is located at chromosome 11q13 between landmarks H4B and D11S2196E. The TM7SF2 gene contains eight coding exons, and their splice site consensus sequences are consistent with AG/GT rule. Northern blot analysis with a cDNA probe corresponding to TM7SF2 revealed varying expression levels of a 1.7-kb transcript in adult human heart, brain, pancreas, lung, liver, skeletal muscle, kidney, ovary, prostate, and testis, but no detectable expression in placenta, spleen, thymus, small intestine, colon (mucosal lining), or peripheral blood leukocytes. The open reading frame in the cDNA sequence codes for a protein of 590 amino acids that is rich in glycine (23%) and arginine (17%) residues in its amino-terminal half and contains seven transmembrane domains in its carboxy-terminal half. The transmembrane region of the putative TM7SF2 protein shows amino acid sequence similarity to those of the lamin B receptor and the C14/C24 sterol reductase.
在本报告中，报道了一个名为TM7SF2的新基因的鉴定及其分子特征。该基因位于先前已定位其他基因的FAU邻近区域（FAUNA）。FAUNA基因簇位于11号染色体q13上，在标记H4B和D11S2196E之间。TM7SF2基因包含8个编码外显子，其剪接位点共有序列符合AG/GT规则。用对应于TM7SF2的cDNA探针进行Northern印迹分析显示，1.7kb转录本在成人心脏、脑、胰腺、肺、肝、骨骼肌、肾、卵巢、前列腺和睾丸中有不同的表达水平，但在胎盘、脾、胸腺、小肠、结肠（黏膜层）或外周血白细胞中未检测到表达。cDNA序列中的开放阅读框编码一个590个氨基酸的蛋白质，该蛋白质在其氨基末端一半富含甘氨酸（23%）和精氨酸（17%）残基，在其羧基末端一半含有7个跨膜结构域。推测的TM7SF2蛋白跨膜区的氨基酸序列与核纤层蛋白B受体和C14/C24固醇还原酶的氨基酸序列相似。
