Hackl T, Zickl M, Dobianer K, Hruza C, Czerwenka K, Spona J
Ludwig Boltzmann Institute of Experimental Endocrinology, Department of Cellular Endocrinology, Vienna, Austria.
Anticancer Res. 1998 Mar-Apr;18(2A):839-42.
Estimation of oestrogen receptors (ER) and progesterone receptors (PgR) by dextran-coated charcoal (DCC) or immunohistochemical methods have become standard practices in the management of breast cancer.
A "multiplex" polymerase chain reaction (PCR) system was developed for quantitative estimation of ER and PgR mRNA in breast tumour specimens.
A statistically significant correlation could be found between the mRNA of the oestrogen and the progesterone receptor (p < or = 0.0001). Protein data defined in classes, compared with mRNA data showed a significant correlation for the oestrogen receptor (p < or = 0.0001) as well as for the progesterone receptor (p < or = 0.046).
Messenger RNA could be determined by the present PCR system in tumours assayed as negative by DCC method. Therefore, this sensitive PCR procedure, which requires small amounts of material may be very useful as a diagnostic test to determine the choice of therapy.
通过葡聚糖包被活性炭（DCC）或免疫组化方法估计雌激素受体（ER）和孕激素受体（PgR）已成为乳腺癌治疗中的标准做法。
开发了一种“多重”聚合酶链反应（PCR）系统，用于定量估计乳腺肿瘤标本中的ER和PgR mRNA。
雌激素和孕激素受体的mRNA之间存在统计学上的显著相关性（p≤0.0001）。与mRNA数据相比，按类别定义的蛋白质数据显示雌激素受体（p≤0.0001）以及孕激素受体（p≤0.046）存在显著相关性。
在DCC法检测为阴性的肿瘤中，本PCR系统可测定信使RNA。因此，这种需要少量材料的灵敏PCR程序作为确定治疗选择的诊断测试可能非常有用。
