Nanda G S, Sun F X, Hofmann G A, Hoffman R M, Dev S B
Genetronics, Inc., San Diego, CA 92121, USA.
Anticancer Res. 1998 Mar-Apr;18(2A):999-1004.
Electroporation therapy (EPT) uses reversible membrane permeabilization of cells by electrical pulses for intracellular delivery of poorly permeating drugs like bleomycin. This treatment modality has been found to significantly increase the cytotoxic effect of the drug to tumor cells.
Tumors of human epidermoid carcinoma of larynx (HEp-2) were xenografted subcutaneously in nude mice. EPT consisted of intratumoral injection of belomycin followed by 6 x 100 microseconds square wave electrical pulses of 1130 Volts. The effect of treatment on tumor growth was monitored over a period of 67 days.
Complete regression of the tumors was observed in 83% of the treated mice 67 days after treatment. These findings were confirmed by histopathological analysis of tumor samples from the treated sites, which showed complete absence of tumor cells.
The results indicate that electroporation therapy is very effective and has potential for treating laryngeal tumors clinically.
电穿孔疗法（EPT）利用电脉冲使细胞的膜通透性可逆，以实现博来霉素等低渗透性药物的细胞内递送。已发现这种治疗方式可显著增强药物对肿瘤细胞的细胞毒性作用。
将人喉表皮样癌（HEp-2）肿瘤皮下移植到裸鼠体内。电穿孔疗法包括瘤内注射博来霉素，随后施加1130伏的6×100微秒方波电脉冲。在67天的时间内监测治疗对肿瘤生长的影响。
治疗67天后，83%的治疗小鼠肿瘤完全消退。对治疗部位肿瘤样本的组织病理学分析证实了这些发现，结果显示完全没有肿瘤细胞。
结果表明电穿孔疗法非常有效，具有临床治疗喉肿瘤的潜力。
