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Enhanced separation of DNA sequencing products by capillary electrophoresis using a stepwise gradient of electric field strength.

作者信息

Inoue H, Tsuhako M, Baba Y

机构信息

Department of Chemistry, Kobe Pharmaceutical University, Japan.

出版信息

J Chromatogr A. 1998 Apr 3;802(1):179-84. doi: 10.1016/s0021-9673(97)01098-4.

DOI:10.1016/s0021-9673(97)01098-4
PMID:9616038
Abstract

The effect of the electric field strength gradient on the separation of DNA sequencing fragments was investigated. We demonstrate that the stepwise gradient of electric field improves the separation of DNA sequencing fragments more than 500 bases in size and diminishes the analysis time for DNA sequencing of lager DNA fragments. The use of the electric field strength gradient induces an increase in the theoretical plate number as predicted by the theoretical formulation discussed in this paper.

摘要

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