Rosenmund C, Stern-Bach Y, Stevens C F
Workgroup Cellular Neurobiology, Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany.
Science. 1998 Jun 5;280(5369):1596-9. doi: 10.1126/science.280.5369.1596.
The subunit stoichiometry of several ligand-gated ion channel receptors is still unknown. A counting method was developed to determine the number of subunits in one family of brain glutamate receptors. Successful application of this method in an HEK cell line provides evidence that ionotropic glutamate receptors share a tetrameric structure with the voltage-gated potassium channels. The average conductance of these channels depends on how many subunits are occupied by an agonist.
几种配体门控离子通道受体的亚基化学计量仍不清楚。开发了一种计数方法来确定一类脑谷氨酸受体中的亚基数量。该方法在人胚肾细胞系中的成功应用提供了证据，表明离子型谷氨酸受体与电压门控钾通道具有四聚体结构。这些通道的平均电导率取决于激动剂占据的亚基数量。
