Bramble L H, Anderson R S
Chesapeake Biological Laboratory, University of Maryland Center for Environmental Science, Solomons, USA.
Dev Comp Immunol. 1998 Jan-Feb;22(1):55-61. doi: 10.1016/s0145-305x(97)00043-8.
If reactive oxygen species (ROS) produced by hemocytes of the eastern oyster, Crassostrea virginica, impart bactericidal activity, exposure of hemocytes to bacteria should result in increased ROS generation. In an earlier study, this hypothesis was tested using luminol- and lucigenin-augmented chemiluminescence (CL) to measure ROS production. The bacterium Listonella anguillarum did not stimulate a net increase in hemocyte-derived CL, and it was suggested that bacterial antioxidants might suppress hemocyte CL. In the present study a comparison was made, under identical assay conditions, of the zymosan- and bacteria-enhanced luminol CL produced by eastern oyster hemocytes and by striped bass (Morone saxatilis) macrophages, for which L. anguillarum has been shown to be a stimulus in CL reactions. The response to zymosan produced by bass phagocytes was two orders of magnitude greater than that generated by eastern oyster hemocytes. Whereas an increase in net ROS production was not evident when oyster hemocytes were exposed to L. anguillarum, significant stimulation of striped bass macrophage-derived CL occurred. These data suggest that striped bass macrophages have a greater capacity to generate ROS than oyster hemocytes, enabling them to surpass the antioxidant capability of L. anguillarum and produce a luminol CL response.
如果美国东海岸牡蛎（Crassostrea virginica）血细胞产生的活性氧（ROS）具有杀菌活性，那么将血细胞暴露于细菌中应该会导致ROS生成增加。在早期的一项研究中，使用鲁米诺和光泽精增强的化学发光（CL）来测量ROS的产生，对这一假设进行了测试。鳗弧菌（Listonella anguillarum）并未刺激血细胞来源的CL净增加，研究表明细菌抗氧化剂可能会抑制血细胞CL。在本研究中，在相同的检测条件下，对美国东海岸牡蛎血细胞和条纹鲈（Morone saxatilis）巨噬细胞产生的酵母聚糖和细菌增强的鲁米诺CL进行了比较，对于条纹鲈巨噬细胞，鳗弧菌已被证明是CL反应的刺激物。条纹鲈吞噬细胞对酵母聚糖产生的反应比美国东海岸牡蛎血细胞产生的反应大两个数量级。当牡蛎血细胞暴露于鳗弧菌时，净ROS生成的增加并不明显，而条纹鲈巨噬细胞来源的CL则受到显著刺激。这些数据表明，条纹鲈巨噬细胞比牡蛎血细胞具有更强的产生ROS的能力，使它们能够超越鳗弧菌的抗氧化能力并产生鲁米诺CL反应。
