Lacoste A, Malham S K, Cueff A, Poulet S A
Station Biologique de Roscoff, CNRS, Université Paris VI, INSU, Place Georges Teissier, BP 74, 29682 Roscoff cedex, France.
Dev Comp Immunol. 2001 May;25(4):285-9. doi: 10.1016/s0145-305x(00)00067-7.
Catecholamines (CA) are known to be present in the microenvironment of molluscan immunocytes. In the present study, experiments were conducted to determine the effects of noradrenaline (NA), the principal CA circulating in bivalve hemolymph, on the luminol-dependent chemiluminescence (CL) of oyster Crassostrea gigas hemocytes. Results show that NA had a dose-dependent inhibitory effect on the CL-response at the physiological concentration of 0.1 microM and above. The alpha-adrenoceptor agonist phenylephrine had no significant effect on the CL-response whereas the beta-adrenoceptor agonist isoproterenol mimicked the inhibitory effects of NA on the CL-response. The beta-adrenoceptor antagonist propanolol, but not the alpha-adrenoceptor antagonist prazosin, prevented the negative effects of NA on the CL-response. Taken together, these results show that beta-adrenergic receptors are present at the surface of oyster hemocytes and allow NA to down-regulate the CL-response.
已知儿茶酚胺（CA）存在于软体动物免疫细胞的微环境中。在本研究中，进行了实验以确定去甲肾上腺素（NA）（双壳贝类血淋巴中循环的主要CA）对牡蛎巨蛎血细胞的鲁米诺依赖性化学发光（CL）的影响。结果表明，在生理浓度0.1微摩尔及以上时，NA对CL反应具有剂量依赖性抑制作用。α-肾上腺素能受体激动剂去氧肾上腺素对CL反应无显著影响，而β-肾上腺素能受体激动剂异丙肾上腺素模拟了NA对CL反应的抑制作用。β-肾上腺素能受体拮抗剂普萘洛尔而非α-肾上腺素能受体拮抗剂哌唑嗪可阻止NA对CL反应的负面影响。综上所述，这些结果表明β-肾上腺素能受体存在于牡蛎血细胞表面，并使NA下调CL反应。
