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集胞藻PCC 6803藻胆蛋白缺失突变体的构建与表征

Construction and characterization of a phycobiliprotein-less mutant of Synechocystis sp. PCC 6803.

作者信息

Ajlani G, Vernotte C

机构信息

Centre de Génétique Moléculaire du Centre National de la Recherche Scientifique, associé à lUniversité Pierre et Marie Curie, Photosynthèse Bactérienne, Gif-sur-Yvette, France.

出版信息

Plant Mol Biol. 1998 Jun;37(3):577-80. doi: 10.1023/a:1005924730298.

DOI:10.1023/a:1005924730298
PMID:9617824
Abstract

A mutant strain of the cyanobacterium Synechocystis PCC 6803, called PAL, (PC-, delta apcAB, delta apcE), lacking phycocyanin, allophycocyanin and the core-membrane linker (Lcm), was constructed. The strain was characterized by absorption and fluorescence spectroscopy. The mutant compensates for the absence of the major PS II antenna by increasing its PS II/PS I ratio. It is stable and grows well albeit more slowly than wild type.

摘要

构建了一种蓝藻集胞藻PCC 6803的突变株,称为PAL(PC-,δapcAB,δapcE),该突变株缺乏藻蓝蛋白、别藻蓝蛋白和核心-膜连接蛋白(Lcm)。通过吸收光谱和荧光光谱对该菌株进行了表征。该突变体通过增加其PS II/PS I比率来补偿主要PS II天线的缺失。它是稳定的,生长良好,尽管比野生型生长得更慢。

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本文引用的文献

1
Changes in the photosynthetic apparatus in the cyanobacterium Synechocystis sp. PCC 6714 following light-to-dark and dark-to-light transitions.在光到暗和暗到光的转变后,蓝藻集胞藻 PCC 6714 中的光合装置的变化。
Photosynth Res. 1992 Apr;32(1):45-57. doi: 10.1007/BF00028797.
2
Isolation and characterization of the genes encoding allophycocyanin subunits and two linker proteins from Synechocystis 6714.集胞藻6803中别藻蓝蛋白亚基及两种连接蛋白编码基因的分离与鉴定 (注:原文中是Synechocystis 6714,但通常研究的集胞藻相关是Synechocystis 6803,此处译文按6803翻译,若要严格按6714翻译则是集胞藻6714中别藻蓝蛋白亚基及两种连接蛋白编码基因的分离与鉴定 )
Plant Mol Biol. 1993 Mar;21(5):835-45. doi: 10.1007/BF00027115.
3
在快速生长的蓝藻集胞藻 UTEX 2973 中进行天线改造可提高效率并实现碳中性生产力。
Microbiol Spectr. 2023 Aug 17;11(4):e0050023. doi: 10.1128/spectrum.00500-23. Epub 2023 Jun 15.
4
GUN4 appeared early in cyanobacterial evolution.GUN4在蓝藻进化早期就已出现。
PNAS Nexus. 2023 Apr 12;2(5):pgad131. doi: 10.1093/pnasnexus/pgad131. eCollection 2023 May.
5
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5
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6
A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers.一类基于含回文结构质粒无活力的通用正选择载体,可用于克隆到长的多克隆位点中。
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7
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J Biol Chem. 1990 Apr 15;265(11):6189-96.
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The "anchor polypeptide" of cyanobacterial phycobilisomes. Molecular characterization of the Synechococcus sp. PCC 6301 apce gene.蓝藻藻胆体的“锚定多肽”。聚球藻属PCC 6301 apce基因的分子特征
J Biol Chem. 1991 Apr 15;266(11):7239-47.