Nickerson P A
Am J Pathol. 1976 Sep;84(3):545-60.
The adrenal cortex of spontaneously hypertensive rats (SHR) has been examined by quantitative morphologic techniques for electron microscopy. The volume and surface area of smooth endoplasmic reticulum and the volume of Golgi apparatuses in zona glomerulosa cells of SHR was significantly greater than those of Wistar-Kyoto strain (W/KY) normotensive controls; the volume of lipid droplets and nucleus was significantly less in SHR than in W/KY animals. A stimulation of the zona glomerulosa in SHR may well be attributable to the elevation in systolic blood pressure. A distinct lipid-free subglomerulosa was observed in the adrenal gland of W/KY rats; the cell volume was similar to that of the zona glomerulosa although the cells showed a significantly greater volume of mitochondria and surface area of mitochondrial membranes and greater volume of smooth endoplasmic reticulum and lysosomes. In the zona fasciculata, cell volume, volumes and surface area of mitochondria and smooth endoplasmic reticulum, and volume of lipid droplets were significantly lower in SHR than in W/KY rats. The volume of the Golgi apparatus was greater in SHR than in W/KY rats. Glycogen particles were observed in focal areas of some zona fasciculata cells. The adrenal cortex of another strain of normotensive Wistar rat (W/CFN) was compared with that of the W/KY and SHR. Although the relative adrenal weights of SHR and W/KY animals were identical, the weight of that in W/CFN was significantly smaller. The volume of the zona glomerulosa of SHR was significantly greater than that of W/KY although the volume of the zona glomerulosa in W/CFN was significantly greater than the other two groups. The volume of nucleus and lipid droplets of zona glomerulosa in W/KY was significantly greater than that in the S/CFN; the volume of the cell, mitochondria, smooth endoplasmic reticulum, lipid droplets, and lysosomes, and the surface area of smooth endoplasmic reticulum and mitochondrial membranes of W/KY animals was significantly greater than those of W/CFN animals. It is concluded that the W/CFN rat is not an appropriate control for spontaneously hypertensive rats.
已采用定量形态学技术对自发性高血压大鼠（SHR）的肾上腺皮质进行电子显微镜检查。SHR球状带细胞中滑面内质网的体积和表面积以及高尔基体的体积显著大于Wistar-Kyoto品系（W/KY）正常血压对照大鼠；SHR中脂滴和细胞核的体积显著小于W/KY动物。SHR球状带的刺激很可能归因于收缩压的升高。在W/KY大鼠的肾上腺中观察到一个明显无脂的球状带下层；尽管这些细胞显示线粒体体积和线粒体膜表面积显著更大，以及滑面内质网和溶酶体体积更大，但其细胞体积与球状带细胞相似。在束状带中，SHR的细胞体积、线粒体和滑面内质网的体积与表面积以及脂滴体积显著低于W/KY大鼠。SHR中高尔基体的体积大于W/KY大鼠。在一些束状带细胞的局部区域观察到糖原颗粒。将另一品系正常血压Wistar大鼠（W/CFN）的肾上腺皮质与W/KY和SHR的进行比较。尽管SHR和W/KY动物的相对肾上腺重量相同，但W/CFN的肾上腺重量显著更小。SHR球状带的体积显著大于W/KY，尽管W/CFN球状带的体积显著大于其他两组。W/KY球状带中细胞核和脂滴的体积显著大于S/CFN；W/KY动物的细胞、线粒体、滑面内质网、脂滴和溶酶体的体积，以及滑面内质网和线粒体膜的表面积显著大于W/CFN动物。得出的结论是，W/CFN大鼠不是自发性高血压大鼠的合适对照。
