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用于制备免疫吸附剂的磁响应性聚丙烯酰胺琼脂糖珠

Magnetically responsive polyacrylamide agarose beads for the preparation of immunoabsorbents.

作者信息

Guesdon J L, Courcon J, Avrameas S

出版信息

J Immunol Methods. 1978;21(1-2):59-63. doi: 10.1016/0022-1759(78)90223-5.

DOI:10.1016/0022-1759(78)90223-5
PMID:96184
Abstract

Glutaraldehyde-activated magnetically responsive polyacrylamide agarose beads have been employed to bind bovine serum albumin and human, sheep and rabbit IgG. These were tested for their effectiveness as immunoabsorbents and were found to allow isolation of pure antibodies in high yields. The use of magnetically responsive beads as the solid support in immunoabsorption procedures renders isolation of antibodies easy and rapid.

摘要

戊二醛活化的磁响应性聚丙烯酰胺琼脂糖珠已用于结合牛血清白蛋白以及人、绵羊和兔的免疫球蛋白G(IgG)。对这些珠作为免疫吸附剂的有效性进行了测试,发现它们能够以高产量分离出纯抗体。在免疫吸附过程中使用磁响应性珠作为固相支持物,使得抗体的分离变得容易且快速。

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Magnetically responsive polyacrylamide agarose beads for the preparation of immunoabsorbents.用于制备免疫吸附剂的磁响应性聚丙烯酰胺琼脂糖珠
J Immunol Methods. 1978;21(1-2):59-63. doi: 10.1016/0022-1759(78)90223-5.
2
Polyacrylamide-agarose beads for the preparation of effective immunoabsorbents.用于制备高效免疫吸附剂的聚丙烯酰胺-琼脂糖珠。
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