Dunlop R H, McEwen S A, Meek A H, Black W D, Friendship R M, Clarke R C
Department of Population Medicine, University of Guelph, Ontario, Canada.
Prev Vet Med. 1998 Mar 27;34(4):265-82. doi: 10.1016/s0167-5877(97)00094-9.
Fecal specimens were composited and a hydrophobic-grid membrane-filter method was used to measure antimicrobial resistance to ampicillin 16 micrograms/ml, carbadox 30 micrograms/ml, gentamicin 4 mu/ml, nitrofurantoin 32 micrograms/ml, spectinomycin 16 micrograms/ml, sulfisoxazole 32 micrograms/ml and tetracycline 8 micrograms/ml among 8119 Escherichia coli isolates from 68 fecal samples collected on 34 farrow-to-finish swine farms marketing over 500 hogs/yr. The overall prevalences of resistance to antimicrobials among these isolates were: ampicillin 29%, carbadox 3.5%, gentamicin 0.6%, nitrofurantoin 27%, spectinomycin 28%, sulfasoxizole 38% and tetracycline 71%. Thirty to seventy-six per cent of the variations in prevalences were explained by between-farm differences.
将来自34个年出栏量超过500头生猪的全进全出式猪场的68份粪便样本中的8119株大肠杆菌分离株的粪便样本进行混合,并采用疏水网格膜过滤法测定其对16微克/毫升氨苄西林、30微克/毫升卡巴多司、4微克/毫升庆大霉素、32微克/毫升呋喃妥因、16微克/毫升壮观霉素、32微克/毫升磺胺异恶唑和8微克/毫升四环素的耐药性。这些分离株中对各抗菌药物的总体耐药率分别为:氨苄西林29%、卡巴多司3.5%、庆大霉素0.6%、呋喃妥因27%、壮观霉素28%、磺胺异恶唑38%、四环素71%。各猪场之间的差异解释了30%至76%的耐药率变化。