Lei K Y, Prasad A S, Bowersox E, Oberleas D
Am J Physiol. 1976 Jul;231(1):98-103. doi: 10.1152/ajplegacy.1976.231.1.98.
The study involved three levels of dietary zinc (deficient, marginal, and adequate) and four hormonal conditions; namely, no steriods, norethindrone, mestranol, and norethindrone plus mestranol. The steroids were incorporated into diets and fed to 11-wk-old female Sprague-Dawley rats. After 10 wk of treatment, various tissues were excised for mineral assays by atomic-absorption spectrophotometry. Both steroids, reduced weight gain. Mestranol depressed plasma zinc, tibia copper and magnesium, and liver iron, but elevated the zinc levels in liver and erythrocytes, plasma copper, liver magnesium and calcium, and iron content of tibia and heart. In general, the effect was most prominent with adequate zinc but diminished in magnitude with the reduction of zinc intake. In addition, norethindrone increased heart iron and tibia calcium. Mestranol appeared to be the main causative factor and may have induced a possible shift of minerals from one pool to another. As expected, zinc deficiency resulted in the reduction of zinc concentrations of plasma, tibia, kidney, and pancreas, and the elevation of copper, iron, magnesium, and calcium concentrations of various tissues.
该研究涉及三种膳食锌水平（缺乏、边缘和充足）以及四种激素状况；即，无类固醇、炔诺酮、炔雌醇甲醚，以及炔诺酮加炔雌醇甲醚。将这些类固醇添加到饲料中，喂食11周龄的雌性斯普拉格-道利大鼠。经过10周的治疗后，切除各种组织，通过原子吸收分光光度法进行矿物质分析。两种类固醇都降低了体重增加。炔雌醇甲醚降低了血浆锌、胫骨铜和镁以及肝脏铁的含量，但提高了肝脏和红细胞中的锌水平、血浆铜、肝脏镁和钙，以及胫骨和心脏的铁含量。一般来说，在锌充足时这种影响最为显著，但随着锌摄入量的减少，其程度会减弱。此外，炔诺酮增加了心脏铁和胫骨钙的含量。炔雌醇甲醚似乎是主要的致病因素，可能导致了矿物质从一个库转移到另一个库。正如预期的那样，锌缺乏导致血浆、胫骨、肾脏和胰腺中锌浓度降低，以及各种组织中铜、铁、镁和钙浓度升高。
