Snashall P D
Am J Physiol. 1977 Jun;232(6):H608-16. doi: 10.1152/ajpheart.1977.232.6.H608.
Subatmospheric pressures were recorded from cotton wicks inserted into Wharton's jelly of human umbilical cords (mean=-7.6+/-5.0 (SD) cmH2O; n=10). Hyaluronate concentrations (mean=0.69+/-0.30 g/100 g) and wick pressures correlated negatively (r=-0.825; P less than 0.01) but calculated hyaluronate osmotic pressures (mean=4.0+/-2.7 cmH2O) were insufficient to explain the full wick pressure. In model systems composed of asbestos fibers (mean radius=0.5 micron) and 0.9% saline, subatmospheric pressures were recorded which varied with the volume of saline added. When hyaluronate (1 g/100 g) was substituted for saline, lower wick pressures were recorded at each volume. The pressure lowering effect of hyaluronate was not seen in a matrix of glass fibers (mean radius=1.0 micron). It is suggested that the effect of hyaluronate in asbestos fibers is due to its immobilization by the matrix and the consequent development of osmotic pressure recorded via the wick. In the coarser glass-fiber matrix, hyaluronate was not immobilized and therefore had no osmotic effect. These experiments support the hypothesis that subatmospheric interstitial pressures result from osmotic pressure of immobilized mucopolysaccharide macromolecules.
从插入人脐带华通氏胶的棉芯记录到低于大气压的压力（平均值 = -7.6±5.0（标准差）cmH₂O；n = 10）。透明质酸盐浓度（平均值 = 0.69±0.30 g/100 g）与棉芯压力呈负相关（r = -0.825；P < 0.01），但计算出的透明质酸盐渗透压（平均值 = 4.0±2.7 cmH₂O）不足以解释全部的棉芯压力。在由石棉纤维（平均半径 = 0.5微米）和0.9%盐水组成的模型系统中，记录到低于大气压的压力，其随添加盐水的体积而变化。当用透明质酸盐（1 g/100 g）替代盐水时，在每个体积下记录到的棉芯压力更低。在玻璃纤维（平均半径 = 1.0微米）基质中未观察到透明质酸盐的降压作用。提示透明质酸盐在石棉纤维中的作用是由于其被基质固定，从而通过棉芯记录到渗透压的产生。在较粗的玻璃纤维基质中，透明质酸盐未被固定，因此没有渗透作用。这些实验支持了以下假说：低于大气压的组织间隙压力是由固定化的粘多糖大分子的渗透压所致。
