Steffen D G, Platner W S
Am J Physiol. 1976 Aug;231(2):650-4. doi: 10.1152/ajplegacy.1976.231.2.650.
Relative percent changes in fatty acid (FA) composition of heart mitochondria and microsomes from 30-day-warm-acclimated and cold-acclimated rats were determined by gas chromatography. Rats in both environmental groups received thyroxine (T4) (1 mg/kg per day) or propylthiouracil (PTU) (5 mg/rat per day) for 10 days prior to sacrifice. Mitochondrial total unsaturated fatty acids (TUFA) decreased in cold-acclimated rats relative to warm-acclimated controls but not in cold-acclimated animals receiving PTU. In mitochondria of the T4-treated warm-acclimated group, the relative content of linoleate decreased and that of stearate increased. Heart microsomes from warm-acclimated rats receiving T4 showed large increases in the percentage content of palmitate and stearate accompanied by reductions in linoleate and arachidonate. In cold-acclimated control rats, the relative content of stearate was elevated in both membrane fractions. The results suggested that thyroid hormones may decrease the TUFA composition of heart subcellular membranes by a relative increase in percent stearic acid and decrease in percent linoleic acid. Similar FA changes in these membranes in cold-acclimated rats may be related to the action of thyroid hormones.
采用气相色谱法测定了30天暖适应和冷适应大鼠心脏线粒体和微粒体中脂肪酸（FA）组成的相对百分比变化。在处死前10天，两个环境组的大鼠均接受甲状腺素（T4）（每天1mg/kg）或丙硫氧嘧啶（PTU）（每天5mg/只）。相对于暖适应对照组，冷适应大鼠线粒体中的总不饱和脂肪酸（TUFA）减少，但接受PTU的冷适应动物中未减少。在T4处理的暖适应组的线粒体中，亚油酸的相对含量降低，硬脂酸的相对含量增加。接受T4的暖适应大鼠的心脏微粒体中，棕榈酸和硬脂酸的百分比含量大幅增加，同时亚油酸和花生四烯酸减少。在冷适应对照大鼠中，两个膜组分中硬脂酸的相对含量均升高。结果表明，甲状腺激素可能通过硬脂酸百分比相对增加和亚油酸百分比降低来降低心脏亚细胞膜的TUFA组成。冷适应大鼠这些膜中类似的FA变化可能与甲状腺激素的作用有关。
