Arora S K, Kapoor G S, Sehgal S
Department of Immunopathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Immunol Cell Biol. 1998 Apr;76(2):186-9. doi: 10.1046/j.1440-1711.1998.00731.x.
Leishmaniasis, a group of visceral and cutaneous diseases, is caused by parasites belonging to one genus comprising approximately 13 different species. Many methods including serological, biochemical and molecular biological techniques have been used by various workers to characterize these different species and isolates of Leishmania, yet there is no single generally accepted criterion. We have identified certain cDNA clones from a library generated from the promastigotes of S1 strain of Leishmania donovani and used them as probes for identification of various isolates of L. donovani and Leishmania tropica. Two of the probes used, E2b (2.0 kb) and E1a (1.3 kb), sequence characterized to be hsp70 of Leishmania, were able to distinguish various isolates of L. donovani from different geographical origins as well as strains of L. donovani from those of L. tropica. Thus, by using recombinant hsp70 cDNA probes, the data indicated that there is a considerable degree of heterogeneity in the heat-shock genes of Leishmania.
利什曼病是一组内脏和皮肤疾病，由属于一个属的寄生虫引起，该属包含约13种不同的物种。不同的研究人员使用了包括血清学、生物化学和分子生物学技术在内的多种方法来鉴定利什曼原虫的这些不同物种和分离株，但尚无单一的普遍接受的标准。我们从杜氏利什曼原虫S1株前鞭毛体构建的文库中鉴定出了某些cDNA克隆，并将其用作探针来鉴定杜氏利什曼原虫和热带利什曼原虫的各种分离株。所使用的两个探针，E2b（2.0 kb）和E1a（1.3 kb），其序列特征为利什曼原虫的hsp70，能够区分来自不同地理来源的杜氏利什曼原虫分离株以及杜氏利什曼原虫菌株与热带利什曼原虫菌株。因此，通过使用重组hsp70 cDNA探针，数据表明利什曼原虫的热休克基因存在相当程度的异质性。
