Berstein L M, Tsyrlina E V, Krjukova O G, Dzhumasultanova S V
Lab. Endocrinology, Prof. N.N. Petrov Research Institute of Oncology, St. Petersburg, Russia.
Cancer Lett. 1998 May 15;127(1-2):95-8. doi: 10.1016/s0304-3835(98)00012-3.
Experiments with a duration of either 3 weeks or 3 months were conducted on female rats with initial body weights of 140-170 g. All animals were divided into four groups, i.e. control, tobacco smoke (TS, a whole-body exposure to the mainstream cigarette smoke), estrogen (EG, diethylstilbestrol) and TS + EG. Injection of EG started on the day of bilateral ovariectomy which was performed in all animals 11 days before the end of the experiment. In short 3-week experiments TS weakly stimulated uterine wet weight and in combination with EG significantly increased the percentage of intact non-unwound DNA in uterine tissue (stimulation stage). In the longer 3-month experiments TS decreased a specific hormonal effect of EG, i.e. the ability to induce progesterone receptors in uterine tissue, and did not interfere with the DNA-damaging effect of diethylstilbestrol. The conclusion was made that different phases exist in hormonal and genotoxic responses to the combined effect of tobacco smoke and estrogens, which may be related to the mechanisms and types of hormonal carcinogenesis.
对初始体重为140 - 170克的雌性大鼠进行了为期3周或3个月的实验。所有动物被分为四组，即对照组、烟草烟雾组（TS，全身暴露于主流香烟烟雾）、雌激素组（EG，己烯雌酚）和TS + EG组。在所有动物实验结束前11天进行双侧卵巢切除，术后当天开始注射EG。在为期3周的短期实验中，TS对子宫湿重有微弱刺激作用，与EG联合使用时显著增加了子宫组织中完整未解开DNA的百分比（刺激阶段）。在为期3个月的长期实验中，TS降低了EG的特定激素效应，即诱导子宫组织中孕酮受体的能力，并且不干扰己烯雌酚的DNA损伤效应。得出的结论是，对烟草烟雾和雌激素联合作用的激素和遗传毒性反应存在不同阶段，这可能与激素致癌的机制和类型有关。
