乳头瘤病毒组装需要主要衣壳蛋白通过两个高度保守的半胱氨酸之间的二硫键形成三聚体。
Papillomavirus assembly requires trimerization of the major capsid protein by disulfides between two highly conserved cysteines.
作者信息
Sapp M, Fligge C, Petzak I, Harris J R, Streeck R E
机构信息
Institute for Medical Microbiology and Hygiene, University of Mainz, D-55101 Mainz, Germany.
出版信息
J Virol. 1998 Jul;72(7):6186-9. doi: 10.1128/JVI.72.7.6186-6189.1998.
Abstract
We have used viruslike particles (VLPs) of human papillomaviruses to study the structure and assembly of the viral capsid. We demonstrate that mutation of either of two highly conserved cysteines of the major capsid protein L1 to serine completely prevents the assembly of VLPs but not of capsomers, whereas mutation of all other cysteines leaves VLP assembly unaffected. These two cysteines form intercapsomeric disulfides yielding an L1 trimer. Trimerization comprises about half of the L1 molecules in VLPs but all L1 molecules in complete virions. We suggest that trimerization of L1 is indispensable for the stabilization of intercapsomeric contacts in papillomavirus capsids.
摘要
我们利用人乳头瘤病毒的病毒样颗粒（VLPs）来研究病毒衣壳的结构和组装。我们证明，主要衣壳蛋白L1的两个高度保守的半胱氨酸中的任何一个突变为丝氨酸，都会完全阻止VLPs的组装，但不会阻止衣壳粒的组装，而其他所有半胱氨酸的突变则不会影响VLP的组装。这两个半胱氨酸形成衣壳间二硫键，产生L1三聚体。三聚化在VLPs中约占一半的L1分子，但在完整病毒体中占所有L1分子。我们认为，L1的三聚化对于乳头瘤病毒衣壳中衣壳间接触的稳定是必不可少的。
相似文献
1
Papillomavirus assembly requires trimerization of the major capsid protein by disulfides between two highly conserved cysteines.
J Virol. 1998 Jul;72(7):6186-9. doi: 10.1128/JVI.72.7.6186-6189.1998.
2
Mutational analysis of human papillomavirus type 16 major capsid protein L1: the cysteines affecting the intermolecular bonding and structure of L1-capsids.
Virology. 2003 Mar 30;308(1):128-36. doi: 10.1016/s0042-6822(02)00099-5.
3
DNA-induced structural changes in the papillomavirus capsid.
J Virol. 2001 Aug;75(16):7727-31. doi: 10.1128/JVI.75.16.7727-7731.2001.
4
Maturation of papillomavirus capsids.
J Virol. 2005 Mar;79(5):2839-46. doi: 10.1128/JVI.79.5.2839-2846.2005.
5
Human papillomavirus 16 minor capsid protein L2 helps capsomeres assemble independently of intercapsomeric disulfide bonding.
Virus Genes. 2005 Dec;31(3):321-8. doi: 10.1007/s11262-005-3250-3.
6
Roles of disulfide linkage and calcium ion-mediated interactions in assembly and disassembly of virus-like particles composed of simian virus 40 VP1 capsid protein.
J Virol. 2001 Jan;75(1):61-72. doi: 10.1128/JVI.75.1.61-72.2001.
7
Intercapsomeric disulfide bonds in papillomavirus assembly and disassembly.
J Virol. 1998 Mar;72(3):2160-7. doi: 10.1128/JVI.72.3.2160-2167.1998.
8
Organization of the major and minor capsid proteins in human papillomavirus type 33 virus-like particles.
J Gen Virol. 1995 Sep;76 ( Pt 9):2407-12. doi: 10.1099/0022-1317-76-9-2407.
9
The L1 major capsid protein of human papillomavirus type 16 variants affects yield of virus-like particles produced in an insect cell expression system.
J Clin Microbiol. 1998 Jul;36(7):2046-51. doi: 10.1128/JCM.36.7.2046-2051.1998.
10
In vitro generation and type-specific neutralization of a human papillomavirus type 16 virion pseudotype.
J Virol. 1996 Sep;70(9):5875-83. doi: 10.1128/JVI.70.9.5875-5883.1996.
引用本文的文献
1
Production of human papillomavirus type 16 virus-like particles in Physcomitrella photobioreactors.
Plant Cell Rep. 2025 Sep 17;44(10):216. doi: 10.1007/s00299-025-03602-x.
2
The Natural History of Cervical Cancer and the Case for MicroRNAs: Is Human Papillomavirus Infection the Whole Story?
Int J Mol Sci. 2024 Dec 3;25(23):12991. doi: 10.3390/ijms252312991.
3
A CRR2-Dependent sRNA Sequence Supports Papillomavirus Vaccine Expression in Tobacco Chloroplasts.
Metabolites. 2023 Feb 21;13(3):315. doi: 10.3390/metabo13030315.
4
Chimeric Human Papillomavirus-16 Virus-like Particles Presenting P18I10 and T20 Peptides from HIV-1 Envelope Induce HPV16 and HIV-1-Specific Humoral and T Cell-Mediated Immunity in BALB/c Mice.
Vaccines (Basel). 2022 Dec 21;11(1):15. doi: 10.3390/vaccines11010015.
5
Cryo EM Analysis Reveals Inherent Flexibility of Authentic Murine Papillomavirus Capsids.
Viruses. 2021 Oct 7;13(10):2023. doi: 10.3390/v13102023.
6
Recent Advances in Our Understanding of the Infectious Entry Pathway of Human Papillomavirus Type 16.
Microorganisms. 2021 Oct 1;9(10):2076. doi: 10.3390/microorganisms9102076.
7
Induction of neutralizing antibodies by human papillomavirus vaccine generated in mammalian cells.
Antib Ther. 2019 Mar 4;2(2):45-53. doi: 10.1093/abt/tbz004. eCollection 2019 Apr.
8
High resolution cryo EM analysis of HPV16 identifies minor structural protein L2 and describes capsid flexibility.
Sci Rep. 2021 Feb 10;11(1):3498. doi: 10.1038/s41598-021-83076-5.
9
Expression of different L1 isoforms of papillomavirus as mechanism to circumvent adaptive immunity.
Elife. 2020 Aug 4;9:e57626. doi: 10.7554/eLife.57626.
10
The Double-Edged Sword Role of Viruses in Gastric Cancer.
Cancers (Basel). 2020 Jun 24;12(6):1680. doi: 10.3390/cancers12061680.
本文引用的文献
1
Intercapsomeric disulfide bonds in papillomavirus assembly and disassembly.
J Virol. 1998 Mar;72(3):2160-7. doi: 10.1128/JVI.72.3.2160-2167.1998.
2
Quantitative disassembly and reassembly of human papillomavirus type 11 viruslike particles in vitro.
J Virol. 1998 Jan;72(1):32-41. doi: 10.1128/JVI.72.1.32-41.1998.
3
Novel structural features of bovine papillomavirus capsid revealed by a three-dimensional reconstruction to 9 A resolution.
Nat Struct Biol. 1997 May;4(5):413-20. doi: 10.1038/nsb0597-413.
4
Crystal structures of murine polyomavirus in complex with straight-chain and branched-chain sialyloligosaccharide receptor fragments.
Structure. 1996 Feb 15;4(2):183-94. doi: 10.1016/s0969-2126(96)00021-4.
5
The structure of simian virus 40 refined at 3.1 A resolution.
Structure. 1996 Feb 15;4(2):165-82. doi: 10.1016/s0969-2126(96)00020-2.
6
Using CLUSTAL for multiple sequence alignments.
Methods Enzymol. 1996;266:383-402. doi: 10.1016/s0076-6879(96)66024-8.
7
Expression of human papillomavirus type 11 L1 protein in insect cells: in vivo and in vitro assembly of viruslike particles.
J Virol. 1993 Apr;67(4):1936-44. doi: 10.1128/JVI.67.4.1936-1944.1993.
8
Self-assembly of human papillomavirus type 1 capsids by expression of the L1 protein alone or by coexpression of the L1 and L2 capsid proteins.
J Virol. 1993 Jan;67(1):315-22. doi: 10.1128/JVI.67.1.315-322.1993.
9
Three-dimensional structure of vaccinia virus-produced human papillomavirus type 1 capsids.
J Virol. 1994 Jul;68(7):4503-5. doi: 10.1128/JVI.68.7.4503-4505.1994.
10
Assembly of the major and the minor capsid protein of human papillomavirus type 33 into virus-like particles and tubular structures in insect cells.
Virology. 1994 May 1;200(2):504-12. doi: 10.1006/viro.1994.1213.
Zotero 插件