DNA诱导的乳头瘤病毒衣壳结构变化。
DNA-induced structural changes in the papillomavirus capsid.
作者信息
Fligge C, Schäfer F, Selinka H C, Sapp C, Sapp M
机构信息
Institute for Medical Microbiology and Hygiene, University of Mainz, D-55101 Mainz, Germany.
出版信息
J Virol. 2001 Aug;75(16):7727-31. doi: 10.1128/JVI.75.16.7727-7731.2001.
Abstract
Human papillomavirus capsid assembly requires intercapsomeric disulfide bonds between molecules of the major capsid protein L1. Virions isolated from naturally occurring lesions have a higher degree of cross-linking than virus-like particles (VLPs), which have been generated in eukaryotic expression systems. Here we show that DNA encapsidation into VLPs leads to increased cross-linking between L1 molecules comparable to that seen in virions. A higher trypsin resistance, indicating a tighter association of capsomeres through DNA interaction, accompanies this structural change.
摘要
人乳头瘤病毒衣壳组装需要主要衣壳蛋白L1分子之间的衣壳间二硫键。从自然发生的病变中分离出的病毒体比在真核表达系统中产生的病毒样颗粒（VLP）具有更高程度的交联。在这里，我们表明将DNA包裹到VLP中会导致L1分子之间的交联增加，这与在病毒体中观察到的情况相当。伴随着这种结构变化，胰蛋白酶抗性增强，这表明通过DNA相互作用，衣壳粒之间的结合更紧密。
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