Fishwick J, Edwards G, Ward S A, McLean W G
Department of Pharmacology and Therapeutics, University of Liverpool, UK.
Neurotoxicology. 1998 Jun;19(3):393-403.
The toxicity caused by the artemisinin derivative dihydroartemisinin in differentiated NB2a neuroblastoma cells was studied by transmission and scanning electron microscopy, Western blotting and immunocytochemistry. Western blotting with monoclonal antibodies failed to detect any specific changes in the cell cytoskeleton, nor were any changes detected by immunocytochemistry. This was consistent with electron microscopy of surviving cell neurites. Transmission electron microscopy revealed that dihydroartemisinin damaged NB2a cell mitochondrial cristae and endoplasmic reticulum. Scanning electron microscopy revealed that dihydroartemisinin depleted the filopodia-like processes projecting from the surface of the cell body and neurites. Some, or all, of these drug-induced changes in differentiating NB2a cells may have a role in the neurotoxicity of artemisinin derivatives.
通过透射电子显微镜、扫描电子显微镜、蛋白质免疫印迹法和免疫细胞化学方法，研究了青蒿素衍生物双氢青蒿素对分化型NB2a神经母细胞瘤细胞的毒性作用。使用单克隆抗体进行蛋白质免疫印迹法未检测到细胞骨架有任何特异性变化，免疫细胞化学方法也未检测到任何变化。这与存活细胞神经突的电子显微镜观察结果一致。透射电子显微镜显示，双氢青蒿素破坏了NB2a细胞的线粒体嵴和内质网。扫描电子显微镜显示，双氢青蒿素使从细胞体和神经突表面伸出的丝状伪足样突起减少。在分化的NB2a细胞中，这些药物诱导的变化中的部分或全部，可能在青蒿素衍生物的神经毒性中发挥作用。
