Kuemmerle N B, Chan W, Krieg R J, Norkus E P, Trachtman H, Chan J C
Department of Pediatrics, Virginia Commonwealth University's Medical College of Virginia, Richmond 23298-0498, USA.
Pediatr Res. 1998 Jun;43(6):791-7. doi: 10.1203/00006450-199806000-00012.
Alpha-tocopherol and fish oil have been reported to modulate the progression of IgA nephropathy in animals and humans. Because fish oil has been reported to exacerbate renal disease in subtotal nephrectomized rats, we investigated the effects of fish oil, with and without alpha-tocopherol, on the course of IgA nephropathy. Experimental IgA nephropathy was induced in male Sprague-Dawley rats, weighing 170-200 g, by oral and i.v. immunization with bovine gamma-globulin for 8 wk. IgA nephropathy was evidenced by hematuria, proteinuria, and IgA deposition in the mesangium. Standard rodent chow, containing 30 IU of alpha-tocopherol/kg of diet, was given to the control and IgA nephropathy rats. Fish oil (20% wt/wt), stripped of alpha-tocopherol preservative, was given to control and a second group of IgA nephropathy rats. Alternatively, corn oil or fish oil was supplemented with alpha-tocopherol at 100 IU/kg of diet and given to the third and fourth groups of IgA nephropathy rats. All animals were killed at 8 wk. Urinary protein excretion, plasma and kidney alpha-tocopherol concentrations, as well as glomerular planar area, and kidney transforming growth factor-beta1 mRNA were analyzed. As determined by reductions in proteinuria, glomerular planar area, and TGF-beta1 mRNA, fish oil with alpha-tocopherol ameliorated the renal injury induced by bovine gamma-globulin, whereas fish oil without alpha-tocopherol did not. Our findings support the importance of alpha-tocopherol, more so than fish oil, in mitigating the injury and promoting repair in experimental IgA nephropathy.
据报道，α-生育酚和鱼油可调节动物和人类IgA肾病的进展。由于据报道鱼油会加重肾次全切除大鼠的肾脏疾病，我们研究了鱼油（含或不含α-生育酚）对IgA肾病病程的影响。通过口服和静脉注射牛γ-球蛋白免疫8周，诱导体重170 - 200 g的雄性Sprague-Dawley大鼠发生实验性IgA肾病。血尿、蛋白尿以及系膜中IgA沉积可证明IgA肾病的存在。给对照组和IgA肾病大鼠喂食含30 IU α-生育酚/kg饲料的标准啮齿动物饲料。将去除α-生育酚防腐剂的鱼油（20%重量/重量）喂给对照组和另一组IgA肾病大鼠。另外，玉米油或鱼油以100 IU/kg饲料补充α-生育酚，并喂给第三组和第四组IgA肾病大鼠。所有动物在8周时处死。分析尿蛋白排泄、血浆和肾脏α-生育酚浓度，以及肾小球平面面积和肾脏转化生长因子-β1 mRNA。通过蛋白尿、肾小球平面面积和TGF-β1 mRNA的降低来确定，含α-生育酚的鱼油可改善牛γ-球蛋白诱导的肾损伤，而不含α-生育酚的鱼油则不能。我们的研究结果支持了在减轻实验性IgA肾病的损伤和促进修复方面，α-生育酚比鱼油更重要。
