Cloning and characterization of an endogenous COS-7 cell thromboxane A2 receptor.

作者信息

Becker K P, Ullian M, Halushka P V

机构信息

Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Division of Clinical Pharmacology, Medical University of South Carolina, Charleston, SC 29425, USA.

出版信息

Biochim Biophys Acta. 1998 May 27;1403(1):109-14. doi: 10.1016/s0167-4889(98)00019-6.

DOI:10.1016/s0167-4889(98)00019-6

PMID:9622606

Abstract

A cDNA for a thromboxane A2 (TXA2) receptor was cloned from an SV40 transformed African Green Monkey kidney cell line (COS-7). The sequence is 98% homologous with the isoform of the human TXA2 receptor and has agonist and antagonist ligand binding characteristics that are not significantly different from the human receptor. Stimulation of the COS-7 cells with the TXA2 receptor agonist, ONO 11113 resulted in a significant increase in cAMP formation that was blocked by a receptor antagonist. The results raise the question of the utility of the COS-7 cell line for studies of cloned and expressed TXA2 receptor signalling mechanisms.

摘要