Jones L L, Kreutzberg G W, Raivich G
Department of Neuromorphology, Max-Planck-Institute of Neurobiology, Am Klopferspitz 18a, 82152 Martinsried, Germany.
Brain Res. 1998 Jun 8;795(1-2):301-6. doi: 10.1016/s0006-8993(98)00325-4.
The transforming growth factor beta's (TGFbeta) are a multipotent family of cytokines with strong immunosuppressive and neurotrophic effects. In the current study, we examined the effect of the TGFbeta's 1, 2 and 3 on the proliferation of ramified microglia cultured on top of a confluent astrocyte monolayer. All three TGFbeta isoforms inhibited proliferation. PCR analysis also showed the presence of mRNA for the TGFbeta receptors type I and II and for all 3 TGFbeta isoforms in microglia, astrocytes and in co-cultures. Moreover, removal of this endogenous TGFbeta activity with antibodies against TGFbeta1 and TGFbeta3 strongly stimulated microglial proliferation. These inhibitory effects on the proliferation of ramified microglia suggest that TGFbeta's may play an important role in the regulation of the microglial population under normal conditions and after injury or disease in the central nervous system.
转化生长因子β（TGFβ）是一类具有强大免疫抑制和神经营养作用的多能细胞因子家族。在本研究中，我们检测了TGFβ1、2和3对在汇合的星形胶质细胞单层上培养的分支状小胶质细胞增殖的影响。所有三种TGFβ同工型均抑制增殖。PCR分析还显示，小胶质细胞、星形胶质细胞及共培养物中存在I型和II型TGFβ受体以及所有三种TGFβ同工型的mRNA。此外，用抗TGFβ1和TGFβ3抗体去除这种内源性TGFβ活性可强烈刺激小胶质细胞增殖。这些对分支状小胶质细胞增殖的抑制作用表明，TGFβ可能在正常条件下以及中枢神经系统损伤或疾病后小胶质细胞群体的调节中发挥重要作用。
