Kato H, Ohashi T, Nakamura N, Nishimura Y, Watari T, Goitsuka R, Tsujimoto H, Hasegawa A
Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.
Vet Immunol Immunopathol. 1995 Oct;48(3-4):221-31. doi: 10.1016/0165-2427(95)05441-8.
Equine interleukin-1 alpha (IL-1 alpha) and IL-1 beta were molecularly cloned to establish a basis for research on inflammatory and immune responses in the horse. Equine peripheral blood mononuclear cells (PBMC) were stimulated with lipopolysaccharide (LPS), and cDNA clones of equine IL-1 alpha and IL-1 beta covering the whole coding sequences were isolated from them. These equine IL-1 alpha and IL-1 beta clones contained open reading frames encoding 271 and 269 amino acids, respectively. The deduced amino acid sequence of equine IL-1 alpha showed 71.6% and 60.2% similarity with that of human and murine IL-1 alpha, respectively. Similarly, the amino acid sequence of equine IL-1 beta showed 66.7% and 61.8% similarity with that of human and murine IL-1 beta, respectively. In both equine IL-1 alpha and IL-1 beta, amino acids at miristoylation sites were well conserved. Dot blot analysis indicated that the expression of IL-1 beta was predominant to that of IL-1 alpha in equine PBMC stimulated with LPS or phorbol myristate acetate.
马白细胞介素-1α（IL-1α）和白细胞介素-1β（IL-1β）被进行分子克隆，为研究马的炎症和免疫反应奠定基础。用脂多糖（LPS）刺激马外周血单核细胞（PBMC），并从中分离出覆盖整个编码序列的马IL-1α和IL-1β的cDNA克隆。这些马IL-1α和IL-1β克隆分别包含编码271和269个氨基酸的开放阅读框。推导的马IL-1α氨基酸序列与人和鼠IL-1α的氨基酸序列分别具有71.6%和60.2%的相似性。同样，马IL-1β的氨基酸序列与人和鼠IL-1β的氨基酸序列分别具有66.7%和61.8%的相似性。在马IL-1α和IL-1β中，肉豆蔻酰化位点的氨基酸都高度保守。斑点印迹分析表明，在用LPS或佛波酯肉豆蔻酸酯刺激的马PBMC中，IL-1β的表达比IL-1α的表达更占优势。
