Malaisse W J, Ladriere L
Laboratory of Experimental Medicine, Brussels Free University, Belgium.
Biochem Mol Biol Int. 1998 May;44(6):1127-31. doi: 10.1080/15216549800202202.
The pentaacetate esters of alpha-D-glucose and beta-L-glucose were recently reported to stimulate insulin release. The possible participation of G-protein-coupled receptors to the insulinotropic action of these esters was investigated in rat pancreatic islets either preincubated with cholera toxin or obtained from animals injected with pertussis toxin. Neither procedure affected adversely the secretory response to the esters in islets incubated in the presence of L-leucine. Thus, in both situations, alpha-D-glucose pentaacetate and, to a lesser extent, beta-L-glucose pentaacetate augmented insulin release evoked by the branched-chain amino acid, whilst beta-L-galactose pentaacetate failed to do so. These findings suggest that G-proteins sensitive to either cholera or pertussis toxins are not involved in one of the two modalities by which these esters are thought to stimulate insulin secretion, namely that independent of the catabolic fate of their hexose moieties.
最近有报道称，α-D-葡萄糖和β-L-葡萄糖的五乙酸酯能刺激胰岛素释放。在预先用霍乱毒素孵育或取自注射百日咳毒素动物的大鼠胰岛中，研究了G蛋白偶联受体对这些酯类促胰岛素作用的可能参与情况。这两种处理方法均未对在L-亮氨酸存在下孵育的胰岛对这些酯类的分泌反应产生不利影响。因此，在这两种情况下，α-D-葡萄糖五乙酸酯以及程度稍轻的β-L-葡萄糖五乙酸酯均能增强支链氨基酸引起的胰岛素释放，而β-L-半乳糖五乙酸酯则不能。这些发现表明，对霍乱毒素或百日咳毒素敏感的G蛋白并不参与这些酯类被认为刺激胰岛素分泌的两种方式之一，即独立于其己糖部分分解代谢命运的那种方式。
