Drew A C, Brindley P J, Lewis F A, Liang Y S, Minchella D J
Molecular Parasitology Unit, Queensland Institute of Medical Research, Brisbane, Australia.
Trop Med Int Health. 1998 May;3(5):373-80. doi: 10.1046/j.1365-3156.1998.00228.x.
Genetic variability within and among four geographical strains of Schistosoma japonicum was examined using a novel repetitive element. The element, termed Sirh1.0, was isolated from genomic DNA of a Philippine strain of S. japonicum using a combination of restriction fragment PCR and band-stab PCR. Sjrh1.0 is a tandemly repeated element, the sequence of which appears to be species-specific, in that it hybridized to DNA from S. japonicum but not to DNA from S. mansoni. Its sequence does not match previously deposited sequences in GenBank. When employed as a probe in Southern hybridization analysis, radiolabelled Sjrh1.0 revealed sex-specific and strain-specific differences in genomic DNA of individual worms. We also found individual genetic variation within geographical isolates of the Asian schistosome.
利用一种新型重复元件检测了日本血吸虫四个地理株内和株间的遗传变异性。该元件称为Sirh1.0，是通过限制性片段PCR和带刺PCR相结合的方法从菲律宾株日本血吸虫的基因组DNA中分离出来的。Sjrh1.0是一个串联重复元件，其序列似乎具有物种特异性，因为它能与日本血吸虫的DNA杂交，但不能与曼氏血吸虫的DNA杂交。其序列与GenBank中先前存入的序列不匹配。当用作Southern杂交分析的探针时，放射性标记的Sjrh1.0揭示了单个虫体基因组DNA中的性别特异性和株特异性差异。我们还发现了亚洲血吸虫地理隔离株内的个体遗传变异。
