Hao X P, Günther T, Roessner A, Price A B, Talbot I C
Academic Department of Pathology, Northwick Park and St Mark's NHS Trust, Harrow, UK.
Mol Pathol. 1998 Feb;51(1):26-9. doi: 10.1136/mp.51.1.26.
To evaluate the respective roles of mdm2 (murine double minute 2) and p53 in the development of colorectal carcinoma.
Formalin fixed, paraffin wax embedded tissues from 72 sporadic adenomas and 55 carcinomas were investigated by means of immunohistochemistry for mdm2 and p53.
mdm2 was expressed weakly in 17 of 72 (23.6%) adenomas and in 14 of 55 (25.4%) carcinomas. p53 was expressed in 19 of 72 (26.4%) adenomas and in 23 of 55 (41.8%) carcinomas. Four adenomas and five carcinomas showed positive staining for both proteins. Overexpression of p53 in adenomas was associated with moderate and severe dysplasia but not with tumour size. No associations were found between the expression of mdm2 and either the degree of dysplasia or tumour size. In carcinomas, neither the expression of p53 nor mdm2 correlated with Dukes's stage, metastasis, or differentiation. No associations were found between the expression of p53 and mdm2 in either adenomas or carcinomas.
Although mdm2 has been reported to be an oncogene, it does not appear to play a major role in the development of colorectal carcinoma.
评估mdm2（小鼠双微体2）和p53在结直肠癌发生发展中的各自作用。
采用免疫组织化学方法对72例散发性腺瘤和55例癌组织的福尔马林固定、石蜡包埋组织进行mdm2和p53检测。
mdm2在72例腺瘤中的17例（23.6%）和55例癌中的14例（25.4%）呈弱表达。p53在72例腺瘤中的19例（26.4%）和55例癌中的23例（41.8%）呈表达。4例腺瘤和5例癌两种蛋白均呈阳性染色。腺瘤中p53的过表达与中重度发育异常相关，但与肿瘤大小无关。mdm2的表达与发育异常程度或肿瘤大小均无相关性。在癌组织中，p53和mdm2的表达均与Dukes分期、转移或分化无关。腺瘤或癌组织中p53和mdm2的表达之间均无相关性。
尽管mdm2已被报道为一种癌基因，但它似乎在结直肠癌的发生发展中不起主要作用。
