Kawasaki Y, Monden T, Morimoto H, Murotani M, Miyoshi Y, Kobayashi T, Shimano T, Mori T
Second Department of Surgery, Osaka University Medical School, Japan.
Am J Clin Pathol. 1992 Feb;97(2):244-9. doi: 10.1093/ajcp/97.2.244.
Expression of p53, a tumor-suppressor gene product, was studied immunohistochemically in microwave-fixed, paraffin-embedded sections of 84 colorectal carcinomas and 44 adenomas. Using a monoclonal antibody (PAb1801), nuclear p53 was detected successfully in 51 of 84 (60.7%) cases of carcinomas, and no stain for p53 was demonstrated in the adjacent normal mucosa. The results in the microwave-fixed, paraffin-embedded sections correlated with those in the frozen sections. The incidence of p53 expression in colorectal carcinomas was high in the cases with distant metastasis, but it was not affected by clinicopathologic features such as tumor size or depth of invasion. In colorectal adenomas, only 4 of 44 (9%) adenomas expressed p53. This expression of p53, however, was restricted to only a few glands within tubular adenomas with mild dysplasia. Thus, p53 protein was expressed preferably by malignant tumors of the colorectum. The present study demonstrated the usefulness of microwave fixation to preserve p53. The immunohistochemical detection of p53 in microwave-fixed, paraffin-embedded sections of colorectal carcinoma and adenoma can provide valuable information about the mechanism of carcinogenesis in colorectal epithelium.
采用免疫组织化学方法，在84例结直肠癌和44例腺瘤的微波固定、石蜡包埋切片中研究了肿瘤抑制基因产物p53的表达。使用单克隆抗体（PAb1801），在84例癌组织中有51例（60.7%）成功检测到细胞核p53，而在相邻正常黏膜中未显示p53染色。微波固定、石蜡包埋切片的结果与冰冻切片的结果相关。结直肠癌中p53表达的发生率在有远处转移的病例中较高，但不受肿瘤大小或浸润深度等临床病理特征的影响。在结直肠腺瘤中，44例腺瘤中只有4例（9%）表达p53。然而，这种p53的表达仅限于轻度发育异常的管状腺瘤内的少数腺体。因此，p53蛋白在结直肠癌中表达更为常见。本研究证明了微波固定对保存p53的有用性。在结直肠癌和腺瘤的微波固定、石蜡包埋切片中对p53进行免疫组织化学检测，可以为结直肠上皮细胞的致癌机制提供有价值的信息。
