Harding T C, Geddes B J, Murphy D, Knight D, Uney J B
Department of Medicine, University of Bristol.
Nat Biotechnol. 1998 Jun;16(6):553-5. doi: 10.1038/nbt0698-553.
We have developed a tetracycline-regulatable adenoviral transfection system that mediates efficient long-term transfer of genes into neuronal cells in vivo. This system allows gene expression to be switched on, then off, and back on again simply by administering or removing doxycycline from the animals' drinking water. This regulatable adenoviral vector system should be of value in behavioral studies and in vivo studies of neuronal gene function, and may further the development of effective gene therapy strategies in the brain.
我们开发了一种四环素调控的腺病毒转染系统，该系统可在体内将基因高效长期转移至神经元细胞。通过在动物饮用水中添加或去除强力霉素，该系统可使基因表达开启、关闭，然后再重新开启。这种可调控的腺病毒载体系统在行为研究和神经元基因功能的体内研究中应具有价值，并可能推动脑部有效基因治疗策略的发展。
