Snyder C, Paugh J R
Department of Optometry, University of Alabama at Birmingham, USA.
Optom Vis Sci. 1998 May;75(5):339-41. doi: 10.1097/00006324-199805000-00023.
The technique of administration and the resulting volume and concentration of the dye delivered to the eye via dye-impregnated paper strips are not standardized or known. The purpose of this study was to use a standard technique for preparation of rose bengal dye from commercially available paper strips to determine the variability in concentration based upon soak time, and to determine the volume delivered by such a technique to a model eye.
Rose bengal filter paper strips were wetted for either 15, 30, or 45 s, and the resulting concentration of dye eluted was determined by spectrophotometry. With the same preparation technique and a 15-s soak time, the strip was applied to wetted filter paper, as a model for the moist surface of the eye. The volume of solution delivered was calculated by weight.
Concentrations of rose bengal for the various soak times were: 15 s: 7.64 (+/- 1.11) by 10(-6) g/ml; 30 s: 9.70 (+/- 1.79) by 10(-6) g/ml; and 45 s: 10.27 (+/- 0.66) by 10(-6) g/ml. The volume delivered to the eye model with this technique was 17.43 microliters (+/- 3.09).
The concentration of rose bengal dye delivered to the ocular surface by means of a wetted strip is relatively low and soak time/technique-dependent. This suggests a possibility that results in clinical studies with rose bengal strip application may be different than if controlled volume applications of commercially available 1% liquid rose bengal dye are used.
通过染料浸渍纸条将染料输送至眼部的给药技术以及由此产生的染料体积和浓度尚未标准化或为人所知。本研究的目的是使用一种标准技术，从市售纸条制备孟加拉玫瑰红染料，以确定基于浸泡时间的浓度变异性，并确定通过该技术输送至模型眼的体积。
将孟加拉玫瑰红滤纸浸湿15、30或45秒，通过分光光度法测定洗脱的染料浓度。采用相同的制备技术和15秒的浸泡时间，将纸条应用于湿滤纸，作为眼部湿润表面的模型。通过重量计算输送的溶液体积。
不同浸泡时间的孟加拉玫瑰红浓度分别为：15秒：7.64（±1.11）×10⁻⁶克/毫升；30秒：9.70（±1.79）×10⁻⁶克/毫升；45秒：10.27（±0.66）×10⁻⁶克/毫升。用该技术输送至眼模型的体积为17.43微升（±3.09）。
通过浸湿纸条输送至眼表的孟加拉玫瑰红染料浓度相对较低，且依赖于浸泡时间/技术。这表明在应用孟加拉玫瑰红纸条的临床研究中，结果可能与使用市售1%液体孟加拉玫瑰红染料的控制体积应用有所不同。
