Thompson C H, Yager J A, Van Rensburg I B
Department of Infectious Diseases, Faculty of Medicine, The University of Sydney, Australia.
Res Vet Sci. 1998 Mar-Apr;64(2):157-61. doi: 10.1016/s0034-5288(98)90012-1.
To determine whether the virus responsible for human molluscum contagiosum (MCV) is the causal agent of a similar disease in horses, in situ hybridisations using cloned fragments of human MCV DNA labelled with digoxigenin were carried out on formalin-fixed biopsy sections of lesions from two horses with molluscum contagiosum-like skin lesions. In both instances there was evidence of specific hybridisation of the labelled probe to target DNA in the sections under high stringency conditions, identified by the development of a deep blue-purple stain in the cytoplasm of cells in the stratum spinosum and stratum granulosum of the lesions and the absence of non-specific hybridisation in adjacent non-lesional areas of the epidermis. These results indicate that on the basis of very close homology of their viral DNA sequences, the causative virus of equine molluscum contagiosum is either identical with, or very closely related to, its human equivalent.
为确定引起人类传染性软疣（MCV）的病毒是否为马身上类似疾病的病原体，使用地高辛标记的人类MCV DNA克隆片段，对两匹患有传染性软疣样皮肤损伤的马的福尔马林固定活检病变切片进行原位杂交。在这两个病例中，在高严格条件下均有证据表明标记探针与切片中的靶DNA发生特异性杂交，表现为病变棘层和颗粒层细胞胞质中出现深蓝色至紫色染色，且表皮相邻非病变区域无非特异性杂交。这些结果表明，基于其病毒DNA序列的高度同源性，马传染性软疣的致病病毒与其人类对应病毒相同或密切相关。
