Gessl A, Waldhäusl W
Department of Medicine III, University of Vienna, Austria.
J Clin Endocrinol Metab. 1998 Jun;83(6):2204-9. doi: 10.1210/jcem.83.6.4889.
To better define prevailing activation of circulating T cell subsets in insulin-dependent diabetes mellitus (IDDM) of recent onset (DM; n = 31; median age +/- SD, 28 +/- 6.9 yr) and of long standing (DML; n = 27; age, 33 +/- 10.4 yr; median duration of disease, 105 months), CD4+ and CD8+ T cells were analyzed to determine their naive and memory subsets as well as their expression of human leukocyte antigen (HLA)-DR, interleukin-2 receptor alpha-chain (CD25), and CD69 by three-color flow cytometry. Twenty-six healthy subjects (HS; age, 32.0 +/- 8.2 yr) served as controls. No deviation was seen in either IDDM group compared to HS in CD25 expression on CD4+ or CD8+ cells or in their CD45RA+ or CD45RA- subsets. HLA-DR expression, however, was increased (P < 0.05) in total CD8+ cells and CD45RA+ cells, with CD45RA- CD8+ cells joining the prevailing pattern only in DML. Among CD4+ cells, increased expression of HLA-DR molecules was restricted to total and CD45RA- cells in DML. CD69 expression did not differ between IDDM and HS, but differed between DML (CD4+, CD8+, and CD45RA- CD4+) and DM only. In conclusion, our data demonstrate that HLA-DR expression in IDDM is restricted to memory cells (CD45RA-) among CD4+ cells in DML and is more markedly confined to naive (CD45RA+) than to memory CD8+ cells, whereas the early activation antigen CD69 is more readily expressed in DML than in DM. The observed activation of circulating T cells suggests an ongoing immune process in IDDM both at clinical manifestation and after long duration.
为了更好地界定近期发病的胰岛素依赖型糖尿病（IDDM；糖尿病组，n = 31；中位年龄±标准差，28±6.9岁）和病程较长的胰岛素依赖型糖尿病（DML；n = 27；年龄，33±10.4岁；中位病程，105个月）中循环T细胞亚群的主要激活情况，通过三色流式细胞术分析CD4⁺和CD8⁺T细胞，以确定其初始和记忆亚群以及人类白细胞抗原（HLA）-DR、白细胞介素-2受体α链（CD25）和CD69的表达。26名健康受试者（HS；年龄，32.0±8.2岁）作为对照。与健康受试者相比，IDDM组中CD4⁺或CD8⁺细胞上CD25的表达及其CD45RA⁺或CD45RA⁻亚群均未见偏差。然而，总CD8⁺细胞和CD45RA⁺细胞中HLA-DR表达增加（P < 0.05），CD45RA⁻CD8⁺细胞仅在DML中呈现主要模式。在CD4⁺细胞中，HLA-DR分子表达增加仅限于DML中的总细胞和CD45RA⁻细胞。IDDM和HS之间CD69表达无差异，但仅在DML（CD4⁺、CD8⁺和CD45RA⁻CD4⁺）和糖尿病组之间存在差异。总之，我们的数据表明，IDDM中HLA-DR表达在DML的CD4⁺细胞中仅限于记忆细胞（CD45RA⁻），并且比记忆CD8⁺细胞更明显地局限于初始（CD45RA⁺）细胞，而早期激活抗原CD69在DML中比在糖尿病组中更容易表达。观察到的循环T细胞激活表明，在IDDM的临床表现期和病程较长后均存在持续的免疫过程。
