水提叶提取物对四氧嘧啶诱导的糖尿病小鼠的影响。
Effects of aqueous leaf extract in alloxan induced diabetic mice.
作者信息
Tuorkey Muobarak J
机构信息
Zoology Department, Division of Physiology, Faculty of Science, Damanhour University , Damanhour, Egypt.
出版信息
Interv Med Appl Sci. 2016 Sep;8(3):109-117. doi: 10.1556/1646.8.2016.3.7.
OBJECTIVE
There is a lack of knowledge regarding the underlying mechanisms of the antidiabetic activity of . This study investigates the antidiabetic effect of and its impact on the immune tolerance.
METHODS
Alloxan-induced diabetes model for mice was used. A dose of 100 mg/kg of Moringa extract was orally administered to diabetic treated mice. Glucose and insulin levels were evaluated to calculate insulin resistance. Total antioxidant capacity (TAC), creatinine, and blood urea nitrogen (BUN) levels were measured. The relative percentage of CD44, CD69, and IFN-γ was investigated by flow cytometry.
RESULTS
In diabetic mice, insulin resistance by homeostasis model assessment of insulin resistance (HOMA-IR) was increased 4.5-fold than in the control group, and HOMA-IR was decreased 1.3-fold in the Moringa treatment group. The level of TAC was declined 1.94-fold in diabetic mice, and increased 1.67-fold in diabetic treated group. In diabetic mice, creatinine and BUN levels were significantly reduced 1.42- and 1.2-fold, respectively, in Moringa treatment mice. The relative percentage of CD44 was not changed in diabetic mice, but the relative percentage of CD69 was found to be increased. INF-γ was decreased 2.4-fold in diabetic mice and elevated in treated groups.
CONCLUSION
Moringa may ameliorate insulin resistance, increase TAC, and improve immune tolerance.
目的
关于[具体物质]抗糖尿病活性的潜在机制，目前尚缺乏相关知识。本研究调查了[具体物质]的抗糖尿病作用及其对免疫耐受的影响。
方法
采用四氧嘧啶诱导的小鼠糖尿病模型。给糖尿病治疗小鼠口服100毫克/千克的辣木提取物。评估血糖和胰岛素水平以计算胰岛素抵抗。测量总抗氧化能力（TAC）、肌酐和血尿素氮（BUN）水平。通过流式细胞术研究CD44、CD69和IFN-γ的相对百分比。
结果
在糖尿病小鼠中，通过稳态模型评估胰岛素抵抗（HOMA-IR）得出的胰岛素抵抗比对照组增加了4.5倍，而在辣木治疗组中HOMA-IR降低了1.3倍。糖尿病小鼠的TAC水平下降了1.94倍，而糖尿病治疗组则增加了1.67倍。在糖尿病小鼠中，辣木治疗小鼠的肌酐和BUN水平分别显著降低了1.42倍和1.2倍。糖尿病小鼠中CD44的相对百分比没有变化，但发现CD69的相对百分比增加。糖尿病小鼠中INF-γ降低了2.4倍，而治疗组中则升高。
结论
辣木可能改善胰岛素抵抗，增加TAC，并提高免疫耐受。
Zotero 插件