Patton A J, Genever P G, Birch M A, Suva L J, Skerry T M
Department of Biology, University of York, United Kingdom.
Bone. 1998 Jun;22(6):645-9. doi: 10.1016/s8756-3282(98)00061-1.
Signaling between the various types of cells found in bone is responsible for controlling the activity of osteoblasts and osteoclasts, and therefore the regulation of bone mass. Our identification of a neuronal glutamate transporter in osteoblasts and osteocytes suggests the possibility that bone cells may use the excitatory amino acid glutamate as a signaling molecule. In these studies we report the expression of different subtypes of glutamate receptors in osteoblasts and osteoclasts in vitro and in vivo. We have identified expression in human and rat bone cells of N-methyl-D-aspartate receptor-1 (NMDAR-1) and 2D subunits and PSD-95, the NMDA receptor clustering protein associated with signaling in the central nervous system. In situ hybridization and immunohistochemistry localized NMDAR-1 expression to osteoblasts and osteoclasts in human tissue sections. These findings strengthen the suggestion that glutamate is involved in signaling between bone cells.
骨骼中发现的各种类型细胞之间的信号传导负责控制成骨细胞和破骨细胞的活性，从而调节骨量。我们在成骨细胞和骨细胞中鉴定出一种神经元谷氨酸转运体，这表明骨细胞可能利用兴奋性氨基酸谷氨酸作为信号分子。在这些研究中，我们报告了谷氨酸受体不同亚型在体外和体内成骨细胞和破骨细胞中的表达。我们已经在人和大鼠骨细胞中鉴定出N-甲基-D-天冬氨酸受体1（NMDAR-1）和2D亚基以及PSD-95的表达，PSD-95是与中枢神经系统信号传导相关的NMDA受体聚集蛋白。原位杂交和免疫组织化学将NMDAR-1的表达定位在人体组织切片中的成骨细胞和破骨细胞上。这些发现进一步支持了谷氨酸参与骨细胞间信号传导的观点。
