[来自商业家禽和鹦鹉的大肠杆菌分离株的维罗毒素产生及黏附基因：通过聚合酶链反应检测]

[Verotoxin production and adhesion genes of E. coli isolates from commercial poultry and psittacines: detection by PCR].

作者信息

Wastlhuber U, Spleiss C, Lohr J E

机构信息

Staatlichen Tierärztlichen Untersuchungsamt Aulendorf.

出版信息

Tierarztl Prax Ausg G Grosstiere Nutztiere. 1998 Feb;26(1):49-52.

PMID:9626748

Abstract

A total of 101 E. coli isolates from commercial poultry, companion psittacine birds and psittacine faecal samples were tested by the PCR technique for the presence of genes coding for verotoxin (slt I and slt II) production and adhesion to intestinal cells (eaeA gene). The O 157:H 7 strain EDL 933 served as positive control. Verotoxin production was assessed in Vero cell cultures. One E. coli isolate showed both alpha-haemolysis and enterohaemolysis (haemolysin production) but no gene for verotoxin production. All other isolates were negative for slt I, slt II and eaeA genes.

摘要

采用聚合酶链反应（PCR）技术，对从商业家禽、伴侣鹦鹉和鹦鹉粪便样本中分离出的总共101株大肠杆菌进行检测，以确定是否存在编码志贺毒素（slt I和slt II）产生以及与肠道细胞黏附（eaeA基因）的基因。O 157:H 7菌株EDL 933用作阳性对照。在Vero细胞培养物中评估志贺毒素的产生。一株大肠杆菌分离株表现出α溶血和肠溶血（产生溶血素），但没有志贺毒素产生基因。所有其他分离株的slt I、slt II和eaeA基因均为阴性。