Na⁺偶联胆汁酸转运体的电生性
Electrogenicity of Na(+)-coupled bile acid transporters.
作者信息
Weinman S A
机构信息
Department of Internal Medicine, University of Texas Medical Branch, Galveston 77555-0641, USA.
出版信息
Yale J Biol Med. 1997 Jul-Aug;70(4):331-40.
Abstract
The Na(+)-bile acid cotransporters NTCP and ASBT are largely responsible for the Na(+)-dependent bile acid uptake in hepatocytes and intestinal epithelial cells, respectively. This review discusses the experimental methods available for demonstrating electrogenicity and examines the accumulating evidence that coupled transport by each of these bile acid transporters is electrogenic. The evidence includes measurements of transport-associated currents by patch clamp electrophysiological techniques, as well as direct measurement of fluorescent bile acid transport rates in whole cell patch clamped, voltage clamped cells. The results support a Na+:bile acid coupling stoichiometry of 2:1.
摘要
钠依赖性胆汁酸转运蛋白NTCP和ASBT分别在很大程度上负责肝细胞和肠上皮细胞中钠依赖性胆汁酸的摄取。本综述讨论了可用于证明电生性的实验方法，并审视了越来越多的证据，即这些胆汁酸转运蛋白中的每一种的偶联转运都是电生性的。证据包括通过膜片钳电生理技术测量转运相关电流，以及在全细胞膜片钳、电压钳制细胞中直接测量荧光胆汁酸转运速率。结果支持钠与胆汁酸的偶联化学计量比为2:1。
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