Takeshita S, Dobashi K, Abe S, Tansho S, Yamaguchi H, Okinaga S, Mori H
Department of Obstetrics and Gynecology, Teikyo University School of Medicine, Tokyo, Japan.
FEMS Immunol Med Microbiol. 1998 Apr;20(4):243-8. doi: 10.1111/j.1574-695X.1998.tb01133.x.
To investigate the effects of peritoneal fluid from patients with endometriosis on mouse peritoneal macrophages (Mphi), peritoneal fluid from endometriosis patients (n = 15) were added to a monolayer of C3H/HeJ mouse peritoneal Mphi. Tumor necrosis factor-producing activity was measured by the L929 assay activated with FK-23 (a preparation of heat-killed Enterococcus faecalis). Tumor necrosis factor-producing activity of C3H/HeJ mouse peritoneal Mphi incubated with peritoneal fluid was suppressed in 14 endometriosis patients. Interestingly, in nine endometriosis patients, tumor necrosis factor-producing activity was much lower than seen with mouse peritoneal Mphi incubated with corticosterone. Peritoneal fluid contains suppressive properties for the activation of peritoneal Mphi, which might allow the implantation of free endometrial cells or the metaplastic phenomena stimulated by retrograde menstruation.
为研究子宫内膜异位症患者的腹腔液对小鼠腹腔巨噬细胞（Mphi）的影响，将15例子宫内膜异位症患者的腹腔液添加到C3H/HeJ小鼠腹腔Mphi单层细胞中。通过用FK - 23（一种热灭活粪肠球菌制剂）激活的L929试验来测量肿瘤坏死因子产生活性。在14例子宫内膜异位症患者中，与腹腔液一起孵育的C3H/HeJ小鼠腹腔Mphi的肿瘤坏死因子产生活性受到抑制。有趣的是，在9例子宫内膜异位症患者中，肿瘤坏死因子产生活性比用皮质酮孵育的小鼠腹腔Mphi低得多。腹腔液对腹腔Mphi的激活具有抑制特性，这可能使游离的子宫内膜细胞得以植入或因逆行月经刺激而发生化生现象。
