Keenan J A, Chen T T, Chadwell N L, Torry D S, Caudle M R
Department of OB/GYN, University of Tennessee Medical Center, Knoxville 37920-6999, USA.
Am J Reprod Immunol. 1995 Dec;34(6):381-5. doi: 10.1111/j.1600-0897.1995.tb00968.x.
The presence of various cytokines in human peritoneal fluid has been incompletely evaluated. Changes in cytokine levels may be related to the development of endometriosis, infertility, and activation of peritoneal macrophages. This study assesses levels of IL-1 beta, IL-2 and TNF- alpha in peritoneal fluid and macrophage conditioned media of women with endometriosis.
Peritoneal fluid was collected from 51 women at the time of diagnostic or operative laparoscopy for benign gynecologic disease. Peritoneal macrophages were isolated, cultured for 24 h, and the culture media collected. IL-1 beta, IL-2, and TNF- alpha levels were determined by commercial ELISA kits.
The mean concentration of IL-1 beta and TNF- alpha was significantly higher in macrophage conditioned media of patients with endometriosis (P < 0.02). However, there were no significant changes in peritoneal fluid cytokine levels. Peritoneal macrophage concentrations were also higher in patients with endometriosis.
This study supports the concept that endometriosis is associated with activation of peritoneal macrophages, and a higher concentration of these cells. This activation is reflected by the increased levels of cytokines found in macrophage conditioned media. The absence of significant changes in peritoneal fluid cytokine levels would seen to indicate that the above derangements are not responsible for the development or progression of endometriosis.
人体腹腔液中多种细胞因子的存在情况尚未得到充分评估。细胞因子水平的变化可能与子宫内膜异位症、不孕症以及腹腔巨噬细胞的激活有关。本研究评估了子宫内膜异位症患者腹腔液和巨噬细胞条件培养基中白细胞介素-1β（IL-1β）、白细胞介素-2（IL-2）和肿瘤坏死因子-α（TNF-α）的水平。
在诊断性或手术性腹腔镜检查时，从51名患有良性妇科疾病的女性中收集腹腔液。分离腹腔巨噬细胞，培养24小时，然后收集培养基。使用商用酶联免疫吸附测定（ELISA）试剂盒测定IL-1β、IL-2和TNF-α的水平。
子宫内膜异位症患者巨噬细胞条件培养基中IL-1β和TNF-α的平均浓度显著更高（P < 0.02）。然而，腹腔液细胞因子水平没有显著变化。子宫内膜异位症患者的腹腔巨噬细胞浓度也更高。
本研究支持这样一种观点，即子宫内膜异位症与腹腔巨噬细胞的激活以及这些细胞的较高浓度有关。这种激活表现为巨噬细胞条件培养基中细胞因子水平的升高。腹腔液细胞因子水平没有显著变化似乎表明上述紊乱与子宫内膜异位症的发生或进展无关。
