小鼠血浆中促黄体生成素的固相测定法。

Solid-phase assay for luteinizing hormone in mouse plasma.

作者信息

Gidley-Baird A A, Bindon B M

出版信息

Aust J Biol Sci. 1976 Mar;29(1-2):105-16.

Abstract

A solid-phase tube assay for measuring LH levels in mouse plasma is described. The assay utilizes an antiserum to ovine LH and ovine LH standards and it measures LH levels in 20 mul of plasma with a sensitivity of less than 0.6 ng/ml. Various parameters affecting the sensitivity and specificity of the assay were investigated. Serial dilutions of plasma from pregnant mice, a pituitary homogenate from mice and plasma from hypophysectomized mice, injected subcutaneously with ovine LH, ran parallel with ovine LH standards in plasma from hypophysectomized mice and plasma with low LH levels from intact mice. Ovine TSH showed about 12% cross reaction in the assay system, whilst rat FSH and prolactin and also ovine FSH, prolactin and GH showed practically no cross reaction. Measurements of plasma LH levels have been made in hypophysectomzied mice after injection with different vehicles containing 10 or 50 mug LH or 50 mug FSH per animal. Daily measurements of LH levels throughout pregnancy in the mouse show a rise in LH level prior to implantationand a further rise around mid-pregnancy which drops off sharply to levels which remain fairly constant until parturition when there is another rise.

摘要

本文描述了一种用于测定小鼠血浆中促黄体生成素（LH）水平的固相管分析法。该分析方法使用抗羊LH抗血清和羊LH标准品，可测定20微升血浆中的LH水平，灵敏度小于0.6纳克/毫升。研究了影响该分析方法灵敏度和特异性的各种参数。对怀孕小鼠的血浆、小鼠垂体匀浆以及皮下注射羊LH的垂体切除小鼠的血浆进行系列稀释，结果显示，这些样品在垂体切除小鼠的血浆和未切除小鼠的低LH水平血浆中与羊LH标准品呈平行关系。在该分析系统中，羊促甲状腺激素（TSH）显示约12%的交叉反应，而大鼠促卵泡激素（FSH）和催乳素以及羊FSH、催乳素和生长激素（GH）几乎没有交叉反应。已对每只动物注射含10或50微克LH或50微克FSH的不同载体后的垂体切除小鼠的血浆LH水平进行了测量。对小鼠整个孕期的LH水平进行每日测量，结果显示，植入前LH水平升高，妊娠中期左右进一步升高，之后急剧下降至相当稳定的水平，直至分娩时再次升高。