Kruip T A, Uilenbroek J T
Tijdschr Diergeneeskd. 1976 Nov 1;101(21):1199-206.
The determination of the concentration of gonadotropic hormones in the peripheral plasma of farm animals by radio-immuno-assay is described. Particular attention was paid to the determination of ovine, bovine and porcine LH using an anti-ovine-LH serum. This serum was found to be specific for LH, and there was no cross-reaction with FSH. Cross-reactions with TSH cannot be ruled out. The usefulness of this antiserum was tested in studying the release of LH during oestrus (in sheep), after injection of LH-RH (in cattle and pigs) and after weaning (in pigs). The results are in accordance with those reported in the literature.
本文描述了通过放射免疫测定法测定家畜外周血浆中促性腺激素浓度的方法。特别关注了使用抗绵羊促黄体生成素(LH)血清来测定绵羊、牛和猪的LH。发现该血清对LH具有特异性,与促卵泡生成素(FSH)无交叉反应。不能排除与促甲状腺激素(TSH)的交叉反应。在研究发情期(绵羊)、注射促性腺激素释放激素(LH - RH)后(牛和猪)以及断奶后(猪)LH的释放情况时,测试了这种抗血清的效用。结果与文献报道一致。