van de Kerkhof P C
Department of Dermatology, University of Nijmegen, The Netherlands.
J Investig Dermatol Symp Proc. 1996 Apr;1(1):78-81.
Vitamin D3 analogs interfere with various aspects of epidermal growth, inflammation, and cellular differentiation. Most data are derived from in vitro studies. In the present review, the in vivo effects of vitamin D3 analogues on the psoriatic plaque are discussed. Calcipotriol, tacalcitol, and calcitriol in ointment modulate aspects of epidermal growth, differentiation, and inflammation. Immunohistochemical studies suggest that the inflammatory changes might be more expressed after treatment with calcitriol and tacalcitol. Flow cytometric quantification of the percentage of cells in SG2M phase and of keratin 10-positive cells revealed that calcipotriol reduced both indices significantly during treatment of psoriatic plaques. Flow cytometric analysis of epidermal cell suspensions using triple labeling for epidermal proliferation, expression of keratin 10, and vimentin permits a quantitative assessment of DNA synthesis selectively in the basal cells of the epidermis, an estimation of the distribution of the basal and suprabasal compartments, and a quantification of the distribution of mesenchymal and nonmesenchymal cells. Using this approach, the interference of tacalcitol with growth control of basal cells was demonstrated. Remarkably, recompartmentalization of basal and suprabasal cells and mesenchymal and nonmesenchymal cells proved to be inconspicuous during this treatment.
维生素D3类似物会干扰表皮生长、炎症和细胞分化的各个方面。大多数数据来自体外研究。在本综述中，讨论了维生素D3类似物对银屑病斑块的体内作用。软膏中的卡泊三醇、他卡西醇和骨化三醇可调节表皮生长、分化和炎症的各个方面。免疫组织化学研究表明，骨化三醇和他卡西醇治疗后炎症变化可能更明显。对处于SG2M期的细胞和角蛋白10阳性细胞百分比进行流式细胞术定量分析发现，在治疗银屑病斑块期间，卡泊三醇显著降低了这两个指标。使用表皮增殖、角蛋白10表达和波形蛋白的三重标记对表皮细胞悬液进行流式细胞术分析，可以选择性地定量评估表皮基底细胞中的DNA合成，估计基底和基底上层隔室的分布，并定量评估间充质和非间充质细胞的分布。使用这种方法，证实了他卡西醇对基底细胞生长控制的干扰。值得注意的是，在这种治疗过程中，基底和基底上层细胞以及间充质和非间充质细胞的重新分布并不明显。
