Komokata T, Nishida S, Ogata S, Hamada N, Ikoma A, Tanaka K, Yoshida H, Taira A
Second Department of Surgery, Kagoshima University Faculty of Medicine, Japan.
In Vivo. 1998 Mar-Apr;12(2):245-51.
The effect of flushing flow rate during multiple organ procurement (MOP) on viability of the liver, pancreas, and intestine were investigated in porcine multivisceral transplantation. Splanchnic organs were flushed in situ with 50 ml/kg of 4 degrees C UW solution via the aorta using a pump at a flow rate of 10, 30, or 50 ml/kg/min. After storage and transplantation, we assessed the clearance of hyaluronic acid (CHA) for hepatic endothelial cells function, liver enzymes, amylase, and histology. Two-day survival was 17% in the 10 ml/kg/min group and 67% in other groups. The former group had inadequate flushing out of the hepatic and intestinal grafts, resulting in aggravation of CHA and intestinal tissue injury. At the flow rate of 30 ml/kg/min, the viability and integrity of all organs were well maintained. We conclude that the optimal flushing flow rate would differ for each organ, therefore the common flow rate acceptable for any of the individual grafts should be applied in MOP.
在猪多脏器移植中，研究了多器官获取（MOP）过程中冲洗流速对肝脏、胰腺和肠道活力的影响。通过主动脉，使用泵以10、30或50 ml/kg/分钟的流速，用50 ml/kg的4℃UW溶液对内脏器官进行原位冲洗。在储存和移植后，我们评估了用于肝内皮细胞功能的透明质酸清除率（CHA）、肝酶、淀粉酶和组织学。10 ml/kg/分钟组的两天存活率为17%，其他组为67%。前一组对肝脏和肠道移植物的冲洗不充分，导致CHA加重和肠道组织损伤。在30 ml/kg/分钟的流速下，所有器官的活力和完整性得到良好维持。我们得出结论，每个器官的最佳冲洗流速会有所不同，因此在多器官获取中应采用任何单个移植物都可接受的通用流速。
